DARS polyclonal antibody
产品名称： DARS polyclonal antibody
英文名称： DARS polyclonal antibody
产品编号： PAB2972
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of DARS.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DARS.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS.
Bonnefond L, Fender A, Rudinger-Thirion J, Giege R, Florentz C, Sissler M.Biochemistry. 2005 Mar 29;44(12):4805-16.
- 2.
- Structure of the N-terminal extension of human aspartyl-tRNA synthetase: implications for its biological function.
Cheong HK, Park JY, Kim EH, Lee C, Kim S, Kim Y, Choi BS, Cheong C.Int J Biochem Cell Biol. 2003 Nov;35(11):1548-57.
- 3.
- Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex.
Sang Lee J, Gyu Park S, Park H, Seol W, Lee S, Kim S.Biochem Biophys Res Commun. 2002 Feb 15;291(1):158-64.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of DARS polyclonal antibody (Cat # PAB2972) in K-562 cell line lysates (35 ug/lane).DARS (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human liver carcinomareacted with DARS polyclonal antibody (Cat # PAB2972), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 1615
- Protein Accession#:
- NP_001340;P14868
- Gene Name:
- DARS
- Gene Alias:
- DKFZp781B11202,MGC111579
- Gene Description:
- aspartyl-tRNA synthetase
- Omim ID:
- 603084
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis. [provided by RefSeq
- Other Designations:
- aspartate tRNA ligase 1, cytoplasmic,cell proliferation-inducing protein 40
- Gene Pathway