Spastin,纤维母细胞表面蛋白/Fibroblast Surface Protein抗体

产品编号HZ-1770R
英文名称Spastin
中文名称纤维母细胞表面蛋白/Fibroblast Surface Protein抗体
别 名Spastin; FSP; Fibroblast surface protein; ADPSP; FSP 2; FSP2; KIAA1083; Spast; Spastic paraplegia 4; SPG 4; SPG4; SPAST_HUMAN.
说 明 书0.1ml 0.2ml
研究领域免疫学 生长因子和激素
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
Spastin,纤维母细胞表面蛋白/Fibroblast Surface Protein抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量68kDa
细胞定位细胞核 细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human FSP
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Spastin,纤维母细胞表面蛋白/Fibroblast Surface Protein抗体PubMedPubMed
产品介绍background:
This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The encoded ATPase may be involved in the assembly or function of nuclear protein complexes. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequences have not been determined. Mutations associated with this gene cause the most frequent form of autosomal dominant spastic paraplegia 4. [provided by RefSeq, Jul 2008]

Subunit:
Homohexamer. Binding to ATP stabilizes the homohexameric form. Binds to microtubules at least in part via the alpha-tubulin and beta-tubulin tails. The hexamer may adopt a ring conformation through which microtubules pass prior to being severed. Does not interact strongly with tubulin heterodimers. Interacts (via MIT domain) with CHMP1B; the interaction is direct. Interacts with ATL1, RTN1, SSNA1 and ZFYVE27.

Subcellular Location:
Membrane; Single-pass membrane protein (Potential). Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Endoplasmic reticulum. Endosome. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Localization to the centrosome is independent of microtubules. Localizes to the midbody of dividing cells, and this requires CHMP1B. Enriched in the distal axons and branches of postmitotic neurons. Isoform 3 is the main endosomal form.

Spastin,纤维母细胞表面蛋白/Fibroblast Surface Protein抗体Tissue Specificity:
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The short isoforms may predominate in brain and spinal cord.

Similarity:
Belongs to the AAA ATPase family. Spastin subfamily.
Contains 1 MIT domain.

Gene ID:
6683

Database links:
Entrez Gene: 6683 Human
Entrez Gene: 50850 Mouse
Entrez Gene: 362700 Rat
Omim: 604277 Human
SwissProt: Q9UBP0 Human
SwissProt: Q9QYY8 Mouse
SwissProt: B2RYN7 Rat
Unigene: 468091 Human

Spastin,纤维母细胞表面蛋白/Fibroblast Surface Protein抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-FSP/Spastin Polyclonal Antibody, Unconjugated(HZ-1770R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining