人解旋酶样转录因子(HLTF)多克隆抗体
Polyclonal Antibody to Helicase Like Transcription Factor (HLTF)
[PROPERTIES]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 500µg/mL
UOM: 100µg
Applications: WB; ICC; IHC-P; IHC-F; ELISA.
[ IMMUNOGEN ]
Immunogen: Recombinant HLTF (Ala837~Lys996) expressed in E.coli.
[ APPLICATIONS ]
Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
[ FORMULATION ]
Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing
0.05% Proclin-300, 50% glycerol.
[STORAGE AND STABILITY] 
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[IDENTIFICATION]

Figure 1. Western Blot
A. Sample: Recombinant HLTF, Human B. Lane1: Human Hela Cells Lane2: Human K562 Cells Lane3: Human jurkat Cells Primary Ab: 3µg/mL Rabbit Anti-Human HLTF Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig

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