Rac-GTP酶激活蛋白1(RACGAP1)重组蛋白

YB293Hu012
Recombinant Rac-GTPase Activating Protein 1 (RACGAP1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
Rac-GTP酶激活蛋白1(RACGAP1)重组蛋白[ PROPERTIES ]
Residues: Met106~Trp539
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: Q9H0H5
Host: E. coli
Subcellular Location: Nucleus, Cytoplasm,
cytoskeleton, spindle, Cytoplasmic vesicle,
secretory vesicle, acrosome, Cleavage furrow,
Midbody, Cell membrane, Peripheral membrane
protein, Cytoplasmic side.
Purity: >90%
Rac-GTP酶激活蛋白1(RACGAP1)重组蛋白Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.9
Predicted Molecular Mass: 78.1kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
Rac-GTP酶激活蛋白1(RACGAP1)重组蛋白MLMCD TSGSIQLSEE QKSALAFLNR GQPSSSNAGN KRLSTIDESG SILSDISFDK
TDESLDWDSS LVKTFKLKKR EKRRSTSRQF VDGPPGPVKK TRSIGSAVDQ GNESIVAKTT
VTVPNDGGPI EAVSTIETVP YWTRSRRKTG TLQPWNSDST LNSRQLEPRT ETDSVGTPQS
NGGMRLHDFV SKTVIKPESC VPCGKRIKFG KLSLKCRDCR VVSHPECRDR CPLPCIPTLI
GTPVKIGEGM LADFVSQTSP MIPSIVVHCV NEIEQRGLTE TGLYRISGCD RTVKELKEKF
LRVKTVPLLS KVDDIHAICS LLKDFLRNLK EPLLTFRLNR AFMEAAEITD EDNSIAAMYQ
AVGELPQANR DTLAFLMIHL QRVAQSPHTK MDVANLAKVF GPTIVAHAVP NPDPVTMLQD
IKRQPKVVER LLSLPLEYW