• 样品类型
  Adherent cells, Suspension cells, Tissue Homogenate
• 检测类型Enzyme activity
• 灵敏度
  < 1 mU/well
• 范围
  2 nmol/well - 10 nmol/well
• 检测时间
  1h 30m
• 种属反应性
  与反应: Mouse, Rat, Human
  预测可用于: all Mammals
• 产品概述

  In Abcam's 6-Phosphofructokinase Activity Assay kit (ab155898), PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. PFK activity assay is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.

• 说明

  Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet). In humans, PFK deficiency causes glycogen storage disease, also called Tarui’s disease, which is characterized by exercise-induced muscle weakness and cramps. On the other hand, increased PFK activity contributes to cancer cell proliferation and tumorigenicity. Early detection of abnormal phosphofructokinase activity is crucial for diagnosis, prediction and therapeutic strategy.

• 经测试应用Functional Studiesmore details

性能

• 存放说明Store at -20°C. Please refer to protocols.

• 组件	标识符	100 tests
  ATP	Orange	1 vial
  NADH Standard	Yellow	1 vial
  PFK Assay Buffer	WM	1 x 27ml
  PFK Developer	Red	1 vial
  PFK Enzyme Mix	Green	1 vial
  PFK Positive Control	Purple	1 vial
  PFK Substrate	Blue	1 vial

• 研究领域
  • Signal Transduction
   
  • Metabolism
   
  • Energy Metabolism

  • Kits/ Lysates/ Other
   
  • Kits
   
  • Cell Metabolism Kits
   
  • Sugar Assays

  • Cancer
   
  • Cancer Metabolism
   
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolic signaling pathways
  • Energy transfer pathways
   
  • Energy Metabolism

应用

6-Phosphofructokinase Activity Assay Kit (Colorimetric) 图像

• 

  Phosphofructokinase activity using ab155898