人肝癌源性生长因子(HDGF)多克隆抗体
Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF)
[PROPERTIES]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200µg/mL
UOM: 100µg
Applications: WB; IHC; ICC; IP.
[ IMMUNOGEN ]
Immunogen: Recombinant HDGF (Tyr10~Leu240) expressed in E.coli.
[ APPLICATIONS ]
Western blotting: 0.5-5µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
[ FORMULATION ]
Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing
0.05% Proclin-300, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant HDGF disposed in loading
buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3.
[STORAGE AND STABILITY] 
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[IDENTIFICATION]

Figure 1. Western Blot
A. Sample: Recombinant HDGF, Human B. Lane1: Human Hela Cells Lane2: Human A549 Cells Lane3: Human Jurkat Cells Lane4: Human 293T Cells Primary Ab: 3µg/mL Rabbit Anti-Human HDGF Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig

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