FITC标记的Ras相关区域家族1A抗体
产品名称： FITC标记的Ras相关区域家族1A抗体
英文名称： Anti-RASSF1A/FITC
产品编号： HZ-20776R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200
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Rabbit Anti-RASSF1A/FITC Conjugated antibody
FITC标记的Ras相关区域家族1A抗体
| 英文名称 | Anti-RASSF1A/FITC |
| 中文名称 | FITC标记的Ras相关区域家族1A抗体 |
| 别 名 | 123F2; AA536941; AU044980; D4Mgi37; MGC94319; NORE2A; PTS; RASSF1; Rassf1A; Rassf1B; Rassf1C; RDA32; REH3P21; Ras association domain family 1A; RASF1_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞凋亡 细胞周期蛋白 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, |
| 产品应用 | Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 39kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 2mg/1ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RASSF1A |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability. Potential tumor suppressor. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. More specifically, RASSF1a inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. It also inhibits the activation of STK3 and STK4 and may play an additional role as a scaffold protein, directing these enzymes to sites of activation. Isoform C has been shown not to perform these roles, and no function has been identified for this isoform. Function: Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK4 during Fas-induced apoptosis. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage. Subunit: Interacts with MAP1S and XPA. Binds to the N-terminal of CDC20 during prometaphase. Binds to STK3/MST2 and STK4/MST1. Isoform A interacts with MOAP1 and E4F1. Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization. Can self-associate. Isoform A interacts with RSSF5 and probably associates with HRAS1 via a RSSF1 isoform A-RSSF5 heterodimer. Part of a complex with MDM2, DAXX, RASSF1 and USP7. Isoform A interacts (via C-terminus) with DAXX (via N-terminus); the interaction is independent of MDM2 and TP53. Isoform C or isoform H interacts (via N-terminus) with DAXX. Isoform A interacts (via N-terminus) with MDM2 (via C-terminus); the interaction is independent of TP53. Subcellular Location: Nucleus. Predominantly nuclear and Cytoplasm. Tissue Specificity: Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells. Similarity: Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Ras-associating domain. Contains 1 SARAH domain. Database links: Entrez Gene: 11186 Human Entrez Gene: 56289 Mouse Omim: 605082 Human SwissProt: Q9NS23 Human SwissProt: Q99MK9 Mouse Unigene: 476270 Human Unigene: 12091 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. RASSF1A是一个肿瘤抑制基因，RASSF1A蛋白的表达能显著抑制肿瘤细胞的生长和转移， 作为一种候选肿瘤抑制基因参与肿瘤的发生发展 ,该基因的失活与其启动子区甲基化有关。 该蛋白在恶性肿瘤的发生发展中发挥重要作用 |
Caspase 9(也称为ICE样凋亡蛋白酶6(ICE LAP6)、凋亡蛋白酶Mch6和凋亡蛋白酶激活因子3(Apaf3))是包含CARD结构域的肽酶家族C14的成员。这种半胱天冬酶作为异源四聚体是活性的，并且已经报道有两种异构体。据报道，脯氨酸酶9约为47 kD。这种胱天蛋白酶存在于细胞质中，并且在激活时，转位到线粒体。Caspase 9参与caspase激活级联，负责细胞凋亡的执行，并切割/激活Caspase 3和Caspase 6。Caspase 9被显性阴性亚型、BclXL、CIAP1、CIAP2、XIAP和Livin抑制。当该caspase被募集到Apaf1/细胞色素c复合物中时，并且在被Apaf1、颗粒酶B、Caspase 3、可能Caspase 8和Caspase 10裂解为大的p37和小的p10亚基之后，该caspase被激活。Caspase 9与BILC7相互作用，已被证明切割PARP和波形蛋白。