E3 ubiquitin ligase,E3泛素连接酶抗体

产品编号HZ-9663R
英文名称E3 ubiquitin ligase
中文名称E3泛素连接酶抗体
别 名C1orf166; E3 ubiquitin ligase; E3 ubiquitin protein ligase MUL1; FLJ12875; GIDE; Growth inhibition and death E3 ligase; MAPL; Mitochondrial anchored protein ligase; Mitochondrial ubiquitin ligase activator of NFKB 1; MUL1; MULAN; Putative NF kappa B activating protein 266; RING finger protein 218; RNF218; RP23-25C1.10-002.
说 明 书0.1ml 0.2ml
研究领域细胞分化 泛素
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Rabbit,
E3 ubiquitin ligase,E3泛素连接酶抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量40kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human E3 ubiquitin ligase/RNF218
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
E3 ubiquitin ligase,E3泛素连接酶抗体PubMedPubMed
产品介绍background:
E3 ubiquitin-protein ligase that plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Function:
Exhibits weak E3 ubiquitin-protein ligase activity, but preferentially acts as a SUMO E3 ligase at physiological concentrations. Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

E3 ubiquitin ligase,E3泛素连接酶抗体Subunit:
Homooligomer. Interacts with MAP3K7/TAK1. Interacts with UBC9. Interacts with and sumoylates DNM1L.

Subcellular Location:
Mitochondrion outer membrane; Multi-pass membrane protein. Peroxisome. Note: Transported in mitochondrion-derived vesicles from the mitochondrion to the peroxisome.

Tissue Specificity:
Widely expressed with highest levels in the heart, skeletal muscle, placenta, kidney and liver. Barely detectable in colon and thymus.

Similarity:
Contains 1 RING-type zinc finger.

Database links:
Entrez Gene: 79594 Human
Entrez Gene: 68350 Mouse
Entrez Gene: 298576 Rat
Omim: 612037 Human
SwissProt: Q969V5 Human
SwissProt: Q8VCM5 Mouse
Unigene: 10101 Human
Unigene: 103413 Mouse

E3 ubiquitin ligase,E3泛素连接酶抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.