B7H4,B7-H4抗体

产品编号HZ-0673R
英文名称B7H4
中文名称B7-H4抗体
别 名B7-H4; B7h4; B7S1; B7x; BC032925; Immune costimulatory protein B7H4; MGC41287; PRO1291; T cell costimulatory molecule B7x; V set domain-containing T cell activation inhibitor 1; VCTN1; VTCN1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 免疫学 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog,
B7H4,B7-H4抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-200 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量28kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human B7H4
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
B7H4,B7-H4抗体PubMedPubMed
产品介绍background:
B7-H4 protein is expressed on the surface of a variety of immune cells and functions as a negative regulator of T cell responses. While B7-H4 mRNA is widely distributed in mouse and human peripheral tissues, cell surface expression of B7-H4 protein is limited and shows an inducible pattern on hematopoietic cells. Putative receptor of B7-H4 can be upregulated on activated T cells. By arresting cell cycle, B7-H4 ligation of T cells has a profound inhibitory effect on the growth, cytokine secretion, and development of cytotoxicity. Administration of B7-H4Ig into mice impairs antigen-specific T cell responses whereas blockade of endogenous B7-H4 by specific monoclonal antibody promotes T cell responses. B7-H4 thus may participate in negative regulation of cell-mediated immunity in peripheral tissues.

Function:
Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

Subcellular Location:
cell membrane; Single-pass type I membrane protein (Potential). Note=Expressed at the cell surface. A soluble form has also been detected.

B7H4,B7-H4抗体Tissue Specificity:
Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis.

Post-translational modifications:
N-glycosylated.

Similarity:
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Gene ID:
79679

Database links:
Entrez Gene: 79679 Human
Entrez Gene: 242122 Mouse
Entrez Gene: 295322 Rat
Omim: 608162 Human
SwissProt: Q7Z7D3 Human
SwissProt: Q7TSP5 Mouse
SwissProt: Q501W4 Rat
Unigene: 546434 Human
Unigene: 137467 Mouse
Unigene: 160956 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.