杀伤细胞免疫球蛋白样受体3DL1(KIR3DL1)重组蛋白

YB562Hu011
Recombinant Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
杀伤细胞免疫球蛋白样受体3DL1(KIR3DL1)重组蛋白[ PROPERTIES ]
Residues: Pro164~Arg437 (Accession # P43629),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Location: Cell membrane; Single-pass
type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 6.5
杀伤细胞免疫球蛋白样受体3DL1(KIR3DL1)重组蛋白Predicted Molecular Mass: 62.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
杀伤细胞免疫球蛋白样受体3DL1(KIR3DL1)重组蛋白MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-PSRLVGQ IHDGVSKANF
SI G P M M L A L A G T Y R C Y G S V T H T P Y Q L S A P S DP L D I V V T G P Y E K P S L S A Q P
GPKVQAGESVTLSCSSRSSY DMYHLSREGG AHERRLPAVR KVNRTFQADF PLGPATHGGT
YRCFGSFRHSPYEWSDPSDP LLVSVTGNPS SSWPSPTEPS SKSGNPRHLH ILIGTSVVII
LFILLLFFLHLWCSNKKNA AVMDQEPAGN RTANSEDSDE QDPEEVTYAQ LDHCVFTQRK
ITRPSQRPKT PPTDTILYTE LPNAKPR