脯氨酰4-羟化酶α多肽Ⅲ(P4Hα3)重组蛋白

YB255Hu011
Recombinant Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
 脯氨酰4-羟化酶α多肽Ⅲ(P4Hα3)重组蛋白10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Glu227~Asp544
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q7Z4N8
Host: E. coli
Subcellular Location: Endoplasmic reticulum
lumen.
Purity: >95%
 脯氨酰4-羟化酶α多肽Ⅲ(P4Hα3)重组蛋白Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.2
Predicted Molecular Mass: 39.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
 脯氨酰4-羟化酶α多肽Ⅲ(P4Hα3)重组蛋白EDAL DHLAFAYFRA GNVSCALSLS REFLLYSPDN KRMARNVLKY ERLLAESPNH
VVAEAVIQRP NIPHLQTRDT YEGLCQTLGS QPTLYQIPSL YCSYETNSNA YLLLQPIRKE
VIHLEPYIAL YHDFVSDSEA QKIRELAEPW LQRSVVASGE KQLQVEYRIS KSAWLKDTVD
PKLVTLNHRI AALTGLDVRP PYAEYLQVVN YGIGGHYEPH FDHATSPSSP LYRMKSGNRV
ATFMIYLSSV EAGGATAFIY ANLSVPVVRN AALFWWNLHR SGEGDSDTLH AGCPVLVGDK
WVANKWIHEY GQEFRRPCSS SPED