Recombinant Human Ubiquitin Activating Enzyme E1 (UBE1) 重组人泛素活化酶E1(UBE1)
产品名称： Recombinant Human Ubiquitin Activating Enzyme E1 (UBE1) 重组人泛素活化酶E1(UBE1)
英文名称： Recombinant Human Ubiquitin Activating Enzyme E1 (UBE1) 重组人泛素活化酶E1(UBE1)
产品编号： 20433ES25
产品价格： 1920.00
产品产地： Yeasen
品牌商标： Yeasen
更新时间： 2023-11-10T15:26:25
使用范围： null
| 规格 | 价格 |
| 25 µg | 1920.0 |
- 联系人 : 李自转
- 地址 : 上海市浦东新区天雄路166弄一号楼三层南单元
- 邮编 : 200030
- 所在区域 : 上海
- 电话 : 139****5640 点击查看
- 传真 : 点击查看
- 邮箱 : lizizhuan@yeasen.com
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产品信息
|
产品名称 |
产品编号 |
规格 |
|
Recombinant Human Ubiquitin Activating Enzyme E1(UBE1) |
20433ES25 |
25 μg |
产品简介
This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions.
产品性质
|
别名(Synonyms) |
UBA1, UBA6 |
|
来源(Source) |
E.coli |
|
分子量 (Molecular Weight) |
Approximately 118 kDa. |
|
氨基酸序列 (AA Sequence) |
MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDT SNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIY DDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR
|
|
外观 |
Liquid |
|
标签(Tag) |
None |
|
纯度(Purity) |
> 95% by SDS-PAG |
|
缓冲液(Buffer) |
50mM PBS, pH7.0, 150mM NaCl, 1mM DTT. |
储存条件
-25~-15℃保存
注意事项
1. 避免反复冻融。
2. 本产品仅作科研用途。
2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
反应缓冲液配方
|
名称 |
组分 |
浓度 |
|
10 × Reaction Buffer（200 ul） |
Tris PH8.0 |
500 mM |
|
DTT |
6 mM |
|
|
ddH2O |
to 200μL |
|
|
10 x ATP-Mg （200 ul）
|
ATP |
100 mM |
|
MgCl2 |
100 mM |
|
|
ddH2O |
to 200μL |
参考阳性反应体系
|
组分 |
体积 (μL) |
|
ddH2O |
to 20 μL |
|
10 × Reaction Buffer |
2 μL |
|
10 × E1 Enzyme |
2 μL |
|
5 × Ubiquitin |
4 μL |
|
10 × ATP-Mg |
2 μL |
|
20 × E2 Enzyme |
1 μL |
|
10 × E3 Ligase Itch (Positive control) |
2 μL |
|
20 × S5a substrate (Positive control) |
1 μL |
Ver.CN20231024
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