FITC标记的跨膜蛋白49抗体
产品名称： FITC标记的跨膜蛋白49抗体
英文名称： Anti-TMEM49/FITC
产品编号： HZ-6740R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-TMEM49/FITC Conjugated antibody
FITC标记的跨膜蛋白49抗体
| 英文名称 | Anti-TMEM49/FITC |
| 中文名称 | FITC标记的跨膜蛋白49抗体 |
| 别 名 | Vacuole membrane protein 1; DKFZP566I133; TDC1; TMEM49; Transmembrane protein 49; vmp1; VMP1_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 心血管 细胞生物 肿瘤细胞生物标志物 细胞自噬 细胞膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Horse, Rabbit, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 46kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TMEM49 (361-406aa) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Vacuole membrane protein 1 (VMP1)/TMEM49 is a transmembrane protein localized to intracellular vacuoles and was discovered as a protein that promotes vacuole formation in acinar cells associated with acute pancreatitis (1). Over-expression of VMP1 promotes vacuole formation and subsequent cell death (1). Subsequent studies have shown that VMP1 expression is induced by starvation and the mTOR inhibitor, rapamycin, and can trigger autophagy (2). VMP1 is targeted, along with LC3, to autophagosome membranes (2). Knockdown of VMP1 can inhibit autophagosome formation (2). VMP1 interacts with Beclin-1, a key autophagy protein that activates the Class III PI3 kinase Vps34, which is regulated by a large network of associated proteins (3). VMP1 functions in the degradation and clearance of zymogen-containing vacuoles during experimental pancreatitis (4). During this process, VMP1 interacts with the ubiquitin protease USP9X, suggesting a possible functional link between the molecular machinery of autophagy and the ubiquitin pathway. Orthologues of VMP1 have been reported in C. elegans (known as EPG-3), Drosophila (known as TANGO-5), and Dictyostelium, and have been shown to play a role in membrane trafficking, orgenelle organization, and autophagy (5-7). Function: Stress-induced protein that, when overexpressed, promotes formation of intracellular vacuoles followed by cell death. May be involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis. Plays a role in the initial stages of the autophagic process through its interaction with BECN1 (By similarity). Involved in cell-cell adhesion. Plays an essential role in formation of cell junctions.Sequence similarities: Belongs to the VMP1 family. Subunit: Belongs to the VMP1 family. Subcellular Location: Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane. Vacuole membrane. Endoplasmic reticulum. Similarity: Belongs to the VMP1 family. Database links: Entrez Gene: 81671 Human Entrez Gene: 75909 Mouse Entrez Gene: 192129 Rat Omim: 611753 Human SwissProt: Q96GC9 Human SwissProt: Q99KU0 Mouse SwissProt: Q91ZQ0 Rat Unigene: 444569 Human Unigene: 708260 Human Unigene: 390398 Mouse Unigene: 109048 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
液泡膜蛋白1（VMP1）/TMEM49是一种定位于细胞内空泡的跨膜蛋白，被发现是促进急性胰腺炎腺泡细胞形成液泡的蛋白（1）。VMP1的过度表达促进空泡形成和随后的细胞死亡（1）。随后的研究表明，VMP1的表达是由饥饿和mTOR抑制剂雷帕霉素引起的，并且可以触发自噬（2）。VMP1与LC3一起被靶向自噬体膜（2）。敲除VMP1可抑制自噬体形成（2）。VMP1与BcIL-1相互作用，BILI-1是激活III类PI3激酶VPS34的关键自噬蛋白，其由相关蛋白（3）的大网络调控。VMP1在实验性胰腺炎过程中含有酶原液泡的降解和清除作用（4）。在这一过程中，VMP1与泛素蛋白酶USP9X相互作用，提示自噬的分子机制与泛素途径之间可能存在功能性联系。VMP1的同源物已报道在线虫（称为EPG-3）、Drosophila（称为Tango5）和盘基网柄菌中，并且已被证明在膜贩运、高尔基体组织和自噬（5-7）中起作用。