ARP2,肌动蛋白相关蛋白2/3抗体

产品编号HZ-12524R
英文名称ARP2
中文名称肌动蛋白相关蛋白2/3抗体
别 名Actin like protein 2; Actin related protein 2; Actin-like protein 2; ACTIN-RELATED PROTEIN 2; ACTR2; ARP2 actin related protein 2 homolog (yeast); ARP2; ARP2_HUMAN; OTTHUMP00000159937; OTTHUMP00000202270.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 信号转导 细胞粘附分子 细胞分化 细胞骨架
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Pig, Cow, Xenopus laevis, Orangutan
ARP2,肌动蛋白相关蛋白2/3抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量45kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ARP2/ARP3
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ARP2,肌动蛋白相关蛋白2/3抗体PubMedPubMed
产品介绍background:
Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping of existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia.

Function:
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.

Subunit:
Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.

Subcellular Location:
Cytoplasm; cytoskeleton. Cell projection.

Similarity:
Belongs to the actin family. ARP2 subfamily.

ARP2,肌动蛋白相关蛋白2/3抗体Gene ID:
10097

Database links:
Entrez Gene: 10097 Human
Entrez Gene: 66713 Mouse
Entrez Gene: 289820 Rat
Entrez Gene: 396147 Chicken
Omim: 604221 Human
SwissProt: P53488 Chicken
SwissProt: P61160 Human
SwissProt: P61161 Mouse
SwissProt: Q5M7U6 Rat
Unigene: 643727 Human
Unigene: 259045 Mouse
Unigene: 102249 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.