NKCC1/SLC12A2,钠钾离子转运蛋白1抗体

产品编号HZ-6945R
英文名称NKCC1/SLC12A2
中文名称钠钾离子转运蛋白1抗体
别 名Basolateral Na-K-Cl symporter; BSC; BSC2; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; mBSC2; NKCC1; S12A2_HUMAN; SLC12A2; sodium-potassium-chloride cotransporter 1; solute carrier family 12 (sodium/potassium/chloride transporters) member 2; Solute carrier family 12 member 2; sy-ns.
说 明 书0.1ml 0.2ml
研究领域肿瘤 心血管 细胞生物 神经生物学 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Chimpanzee,
NKCC1/SLC12A2,钠钾离子转运蛋白1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量133kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human NKCC1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
NKCC1/SLC12A2,钠钾离子转运蛋白1抗体PubMedPubMed
产品介绍background:
Na-K-Cl cotransporters (NKCC) are channel proteins that aid in the transcellular movement of chloride across both secretory and absorptive epithelia. NKCC1 is expressed in muscle cells, neurons, and red blood cells. In the basolateral membrane of secretory epithelia, NKCC1 mediates active chloride secretion. The gene encoding human NKCC1 maps to chromosome 5q23.3. In mice, disruption of the NKCC1 gene leads to deafness and impaired balance. NKCC2 is specifically expressed in the kidney where it mediates active reabsorption of sodium chloride in the thick ascending limb of the loop of Henle. NKCC2 is sensitive to the clinically important diuretics furosemide and bumetanide. The gene encoding human NKCC2 maps to chromosome 15q15-q21 and mutations in this gene lead to Bartter’s syndrome, an inherited hypokalaemic alkalosis. NCCT is a thiazide-sensitive Na-Cl cotransporter that is primarily expressed in the distal convoluted tubule of the kidney where it accounts for a significant fraction of net renal sodium reabsorption. The gene for human NCCT map to chromosome 16q13. Mutations in the gene encoding NCCT cause Gitelman’s syndrome, a subset of Bartter’s syndrome.

Function:
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in many tissues.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity:
Belongs to the SLC12A transporter family.

Database links:
Entrez Gene: 471620 Chimpanzee
Entrez Gene: 286845 Cow
Entrez Gene: 481490 Dog
Entrez Gene: 6558 Human
Entrez Gene: 20496 Mouse
Entrez Gene: 100516960 Pig
Entrez Gene: 83629 Rat
Omim: 600840 Human
SwissProt: P55011 Human
SwissProt: P55012 Mouse
Unigene: 162585 Human
Unigene: 712970 Human
Unigene: 399997 Mouse
Unigene: 11523 Rat

NKCC1/SLC12A2,钠钾离子转运蛋白1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.