植物蛋白提取试剂盒
产品名称： 植物蛋白提取试剂盒
英文名称： 1 | PEB (4x) | protein extraction buffer
产品编号： AS08300
产品价格： 0
产品产地： 瑞典
品牌商标： agrisera
更新时间： null
使用范围： null
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植物蛋白提取试剂盒， 1 | PEB (4x) | protein extraction buffer
货号： AS08300
background | PEB is an extraction buffer for disruption and solubilisation of total protein from plant tissue and algal cells. The use of the anionic detergent LDS together with the recommended procedure (combination of sonication and freeze/thaw cycles) has been shown to increase the quantity of solubilised and non-degraded proteins when compared to other methods of cell disruption (see reference). The estimated hands-on time for the recommended procedure is 20-30 minutes for 1-2 samples. Expected yields will be 1.5-6 µg/µl total protein (recovered from standard procedure) depending on the starting material.
quantity | 5x2 ml (4x stock); allows up to 75 isolations of plant material (using 500 µl 1x PEB for 100 mg fresh weight) or 190 isolations of algal material (using 200 µl 1x PEB for cell amounts corresponding to 4-10 ug total chlorophyll)
specificity | PEB has been tested on a wide range of species and tissues from higher plants, mosses, lichens, algae, diatoms, dinoflagellates, and cyanobacteria. Extracts may be quantified using detergent (LDS) compatible methods, and have been shown to give highly reproducible and quantitative results in subsequent SDS PAGE gel electrophoresis, Western Blotting, and immunoprecipitation. Most of Agrisera commercial antibodies are tested on plant or algal samples extracted with this buffer. An example can be found www.bio-goods.com
For help with protein extraction from algal and cyanobacterial cells you are welcome to www.bio-goods.com.
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Application example A - Agrisera PEB - protein extraction buffer B - SDS containing protein extraction buffer PEB is ready to use directly when taken from refridgerator: due to presence of LDS Agrisera buffer does not precipitate as compared to SDS containing extraction buffers. |
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Agrisera tips for a successful protein extraction from plant and algal samples
► use a protease inhibitor always made from a fresh stock
► keeping a sample volume in a range of 0.2-0.5 ml will contribute to better extraction results (per 100 mg of plant tissue or 4-10 ug of chlorophyll for algal samples
► keep material cool at all times
► for photosynthetic tissues/algal samples intense green pellet indicates that extraction is not optimal and needs to be repeated
► protein extracts should be stored at -80°C aliquoted and used only ONCE
► reducing agent should be added before heating just before loading on a gel
► Agrisera protein extraction buffer - especially prepared for isolation of plant and algal proteins can be used