Monoclonal Antibodies
Anti-Human Leptin (Monoclonal Antibody)





 





		Preparation :
Produced in BALB/c mice using highly pure (>98%) recombinant human Leptin as the immunizing antigen.  This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.



		Catalog #:
500-M27



		Source :
Mice



		Formulation :
A sterile filtered antibody solution was lyophilized from PBS.



		Stability :
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. Frozen aliquots are stable for at least six weeks at 2-8oC or for at least six months at -20oC. Avoid repeated freeze-thaw cycles.


Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml.



		Applications :
 



		Neutralization 
Data Not Available.



		Elisa 
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect recombinant human Leptin when used with PeproTech's biotinylated antigen affinity purified anti-human Leptin (500-P86Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.



		Western Blot 
To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2.0-4.0 ng/lane, under non-reducing conditions.


Country of Origin :
USA
















        Size B
500μg
$150 
 


 















Special 
                    Sizes: 
 Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request.





Usage:
For Research Use Only.  Not for use in diagnostic or therapeutic procedures.