• 经测试应用IHC-Fr, WB, ICC/IF, IHC-Pmore details
• 种属反应性
  与反应: Mouse, Rat, Human
• 免疫原

  Synthetic peptide within Human SNAIL+SLUG aa 236-264. The exact sequence is proprietary.
  Database link: O95863

• 阳性对照
  • Mouse heart tissue extracts

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液pH: 7.3
  Preservative: 0.02% Sodium azide
  Constituents: 50% Glycerol, 49% PBS
• 浓度Batch dependent within range: 100 µl 浓度为 1 - 7.11 mg/ml 
  

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• 纯度Immunogen affinity purified
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Epigenetics and Nuclear Signaling
   
  • Transcription
   
  • Domain Families
  • Zinc Finger

  • Cancer
   
  • Oncoproteins/suppressors
   
  • Oncoproteins
   
  • Transcription factors

  • Cardiovascular
   
  • Heart
   
  • Cardiogenesis
   
  • Transcription factors/regulators

应用

Anti-SNAIL+SLUG antibody 图像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL+SLUG antibody (ab180714)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 200X.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL+SLUG antibody (ab180714)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 400X.

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  Immunocytochemistry/ Immunofluorescence - Anti-SNAIL+SLUG antibody (ab180714)This image is courtesy of an anonymous Abreview

  ab180714 staining SNAIL in human MCF10A cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 3.7% PFA in PBS for 15 minutes and blocked with 0.1% Milk + 0.1% Tween in PBS for 30 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in PBS + 0.1% Tween) for 16 hours at 4°C. An Alexa Fluor^® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - Anti-SNAIL+SLUG antibody (ab180714)This image is courtesy of an anonymous Abreview

  ab180714 staining SNAIL in mouse embryonic heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours at 4°C. An undiluted Alexa Fluor^® 488-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

  See Abreview

• 

  Western blot - Anti-SNAIL+SLUG antibody (ab180714)
  Anti-SNAIL+SLUG antibody (ab180714) at 1/500 dilution + Mouse heart tissue extracts
  
  Predicted band size : 29 kDa
  
• 

  Western blot - Anti-SNAIL+SLUG antibody (ab180714)
  
  
  Predicted band size : 29 kDa
  

  Lane 1: Snail recombinant 0.1 ug (55 kDa)

  Lane 2: Slug recombinant 0.1 ug  (56 kDa)