EIF3S4,EIF3S4蛋白抗体

产品编号HZ-14545R
英文名称EIF3S4
中文名称EIF3S4蛋白抗体
别 名44kDa; Anti EIF3S4; eIF 3 delta; eIF 3 RNA binding subunit; eIF-3 RNA-binding subunit; eIF-3-delta; eIF3 delta; eIF3 p42; eIF3 p44; eIF3g; EIF3G_HUMAN; EIF3S4; EIF3S4; ; Eukaryotic translation initiation factor 3; Eukaryotic translation initiation factor 3 RNA binding subunit; Eukaryotic translation initiation factor 3 RNA-binding subunit; Eukaryotic translation initiation factor 3 subunit 4; Eukaryotic translation initiation factor 3 subunit 4 delta 44 kD; Eukaryotic translation initiation factor 3 subunit G; Eukaryotic translation initiation factor 3 subunit p42; Subunit 4 (delta 44kD); Subunit 4 delta.
说 明 书0.1ml 0.2ml
研究领域细胞生物 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse,
EIF3S4,EIF3S4蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量36kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human EIF3S4
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
EIF3S4,EIF3S4蛋白抗体PubMedPubMed
产品介绍background:
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that lead to 80S ribosomal assembly and, ultimately, translation. The eukaryotic initiation factor-3 (eIF3) scaffolding structure is the largest of the eIF complexes and includes eIF3 alpha, eIF3 beta, eIF3 gamma, eIF3 delta, all of which function to control the assembly of the 40S ribosomal subunit. Association of eIF3 proteins with the 40S ribosomal subunit stabilizes eIF2-GTP-Met-tRNAiMet complex association and mRNA binding, and promotes dissociation of 80S ribosomes into 40S and 60S subunits, thereby promoting the assembly of the pre-initiation complex. Overexpression of eIF3 proteins is common in several cancers, suggesting a role for eIF3 proteins in tumorigenesis.

Function:
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.

Subcellular Location:
Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Colocalizes with AIFM1 in the nucleus and perinuclear region.

EIF3S4,EIF3S4蛋白抗体Post-translational modifications:
Phosphorylated. Phosphorylation is enhanced upon serum stimulation.

DISEASE:

Similarity:
Belongs to the eIF-3 subunit G family.
Contains 1 RRM (RNA recognition motif) domain.

Gene ID:
8666

Database links:
Entrez Gene: 8666 Human
Entrez Gene: 53356 Mouse
Entrez Gene: 298700 Rat
Entrez Gene: 393974 Zebrafish
Omim: 603913 Human
SwissProt: Q3ZC12 Cow
SwissProt: O75821 Human
SwissProt: Q9Z1D1 Mouse
SwissProt: Q5RK09 Rat
SwissProt: Q6DRC4 Zebrafish
Unigene: 529059 Human
Unigene: 427309 Mouse
Unigene: 107880 Rat
Unigene: 161 Zebrafish

EIF3S4,EIF3S4蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-EIF3S4 Polyclonal Antibody, Unconjugated(HZ-14545R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining