LC3抗体
Applications
| In-house applications |
ELISA, IHC, FC, IP, IF, WB Application not tested? Please see our Guarantee. |
| Published applications |
IF, WB |
Publications |
2 citations –view here |
| Species specificity |
Human, Mouse, Rat; other species not tested. |
| Published Species |
Human |
|
Images
Prev123Next
 Chloroquine treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 14600-1-AP( LC3 Antibody) at dilution of 1:1000 Immunofluorescent analysis of Starvation treated HEK-293 cells using 14600-1-AP (LC3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 20 mM chloroquine was used to block the autophagy flux. Immunofluorescent analysis of Chloroquine treated HepG2 cells and HepG2 cells using 14600-1-AP( LC3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunohistochemical of paraffin-embedded human gliomas using 14600-1-AP (LC3 antibody) at dilution of 1:50 (under 40x lens). Immunohistochemical of paraffin-embedded human gliomas using 14600-1-AP(LC3 antibody) at dilution of 1:50 (under 10x lens) 1X10^6 HeLa cells were stained with 0.2ug LC3 antibody (14600-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. IP Result of anti-LC3 (IP:14600-1-AP, 3ug; Detection:14600-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
|
| Positive WB detected in |
Chloroquine treated HEK-293 cells, HEK-293 cells, HeLa cells, human skeletal muscle tissue, MCF7 cells, mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse testis tissue, NIH/3T3 cells, rat brain tissue |
| Positive IP detected in |
Mouse brain tissue |
| Positive IHC detected in |
Human gliomas tissue, human breast cancer tissue, human testis tissue |
| Positive IF detected in |
Starvation treated HEK-293 cells, Chloroquine treated HepG2 cells |
| Positive FC detected in |
HeLa cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:20-1:200 |
LC3抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information LC3抗体
| Immunogen |
LC3 fusion protein ag6144 |
Full name |
Microtubule-associated protein 1 light chain 3 beta |
Calculated molecular
weight |
15 kDa |
Observed molecular
weight |
16-18 kDa |
GenBank accession
number |
BC067797 |
Gene ID (NCBI) |
81631 |
| Gene symbol |
MAP1LC3B |
Synonyms |
LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B |
Background LC3抗体
|
Map1LC3, also known as LC3, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18-kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 16-kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C. LC3抗体
|
XY647Hu22 Skl同源物1(Skl1)单克隆抗体 Monoclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Skl1) Homo sapiens (Hukl)
XY648Hu22 Skl同源物7(Skl7)单克隆抗体 Monoclonal Antibody to Mothers Against Decapentaplegic Homolog 7 (Skl7) Homo sapiens (Hukl)
XY651Hu22 透明质酸结合蛋白1(HABP1)单克隆抗体 Monoclonal Antibody to Hyaluronan Binding Protein 1 (HABP1) Homo sapiens (Hukl)
XY653Hu22 骨形成蛋白1(BMP1)单克隆抗体 Monoclonal Antibody to Bone Morphogenetic Protein 1 (BMP1) Homo sapiens (Hukl)
XY656Hu22 二胺氧化酶(DAO)单克隆抗体 Monoclonal Antibody to Diamine Oxidase (DAO) Homo sapiens (Hukl)
XY657Hu22 谷胱甘肽S转移酶μ2(GSTμ2)单克隆抗体 Monoclonal Antibody to Glutathione S Transferase Mu 2, Muscle (GSTm2) Homo sapiens (Hukl)
XY659Hu22 谷胱甘肽S转移酶α4(GSTα4)单克隆抗体 Monoclonal Antibody to Glutathione S Transferase Alpha 4 (GSTa4) Homo sapiens (Hukl)
XY662Hu22 白介素7(IL7)单克隆抗体 Monoclonal Antibody to Interleukin 7 (IL7) Homo sapiens (Hukl)
XY667Hu22 葡萄糖激酶(GCK)单克隆抗体 Monoclonal Antibody to Glucokinase (GCK) Homo sapiens (Hukl)
XY668Hu22 血管生成素4(ANGPT4)单克隆抗体 Monoclonal Antibody to Angiopoietin 4 (ANGPT4) Homo sapiens (Hukl)
XY669Hu22 激肽释放酶11(KLK11)单克隆抗体 Monoclonal Antibody to Kallikrein 11 (KLK11) Homo sapiens (Hukl)
XY672Hu22 Slit同源物2(Slit2)单克隆抗体 Monoclonal Antibody to Slit Homolog 2 (Slit2) Homo sapiens (Hukl)
XY673Hu22 多巴胺受体D2(DRD2)单克隆抗体 Monoclonal Antibody to Dopamine Receptor D2 (DRD2) Homo sapiens (Hukl)
XY675Hu22 凝血因子Ⅴ(F5)单克隆抗体 Monoclonal Antibody to Coagulation Factor V (F5) Homo sapiens (Hukl)
XY677Hu22 凝血因子Ⅻ(F12)单克隆抗体 Monoclonal Antibody to Coagulation Factor XII (F12) Homo sapiens (Hukl)
