AFG3L2抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
AFG3L2抗体

AFG3L2抗体

商家询价

产品名称： AFG3L2抗体

英文名称： AFG3L2Antibody

产品编号： XY14631-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 AFG3L2抗体

Applications
In-house applications ELISA, WB, IHC, IF, IP     Application not tested? Please see our Guarantee.
Published applications WB Publications 3 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human, mouse
Images
Prev12NextView more validation data
  • 14631-1-AP;HeLa cellHeLa cells were subjected to SDS PAGE followed by western blot with 14631-1-AP(AFG3L2 antibody) at dilution of 1:800
  • 14631-1-AP;H-kidneyImmunohistochemical of paraffin-embedded human kidney using 14631-1-AP(AFG3L2 antibody) at dilution of 1:50 (under 10x lens)
  • 14631-1-AP;H-kidneyImmunohistochemical of paraffin-embedded human kidney using 14631-1-AP(AFG3L2 antibody) at dilution of 1:50 (under 40x lens)
  • 14631-1-AP;HepG2Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
  • 14631-1-AP;mouse kidney tissueIP Result of anti-AFG3L2 (IP:14631-1-AP, 4ug; Detection:14631-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.
Positive WB detected in HeLa cells, HEK-293 cells, human brain tissue, mouse brain tissue, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue
Positive IP detected in Mouse kidney tissue
Positive IHC detected in Human kidney tissue
Positive IF detected in HepG2 cells
Recommended dilution WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:10-1:100
 AFG3L2抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen AFG3L2 fusion protein ag6209 Full name AFG3 ATPase family gene 3-like 2 (yeast)
Calculated molecular
weight		 88 kDa Observed molecular 
weight		 80-90 kDa
GenBank accession 
number		 BC065016 Gene ID (NCBI) 10939
Gene symbol AFG3L2 Synonyms AFG3 like protein 2, AFG3L2, FLJ25993, Paraplegin like protein
Background AFG3L2抗体

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5). AFG3L2抗体
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