胎盘碱性磷酸酶样蛋白2(ALPPL2)重组蛋白

YB649Hu013
Recombinant Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 胎盘碱性磷酸酶样蛋白2(ALPPL2)重组蛋白[ PROPERTIES ]
Residues: Ile20~Arg333 (Accession # P10696),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Cell membrane;
Lipid-anchor, GPI-anchor. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 1mM DTT, 5% trehalose, 0.01%
sarcosyl and preservative.
 胎盘碱性磷酸酶样蛋白2(ALPPL2)重组蛋白Predicted isoelectric point: 6.9
Predicted Molecular Mass: 35.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
 胎盘碱性磷酸酶样蛋白2(ALPPL2)重组蛋白[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-I IPVEEENPDF WNRQAAEALG AAKKLQPAQT AAKNLIIFLG
DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK TYSVDKHVPD SGATATAYLC
GVKGNFQTIG LSAAARFNQC NTTRGNEVIS VMNRAKKAGK SVGVVTTTRV QHASPAGAYA
HTVNRNWYSD ADVPASARQE GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY
SQ G G T R L D GK NLV QEW LAK H QGA RY VW N RT EL L QAS L DP S VT H LMG L FE P
GDMKYEIHRD STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGR
[ REFERENCES ]
1. Millan J.L., Manes T. (1988) Proc. Natl. Acad. Sci. U.S.A. 85:3024-3028. 2. Watanabe S., et al. (1989) J. Biol. Chem. 264:12611-12619. 3. Gum J.R. Jr., et al. (1990) Cancer Res. 50:1085-1091. 4. Lowe M.E., Strauss A.W. (1990) Cancer Res. 50:3956-3962.