Ankrd26 polyclonal antibody
产品名称： Ankrd26 polyclonal antibody
英文名称： Ankrd26 polyclonal antibody
产品编号： PAB10090
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
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- 所在区域 : 台湾
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of Ankrd26.
- Immunogen:
- A synthetic peptide corresponding to amino acids 1-15 of mouse Ankrd26.
- Host:
- Rabbit
- Reactivity:
- Bovine, Human, Mouse, Rat
- Specificity:
- This affinity-purified antibody is directed against mouse Ankrd26 protein.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:20000-1:80000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Western Blot
- Western blot using Ankrd26 polyclonal antibody (Cat # PAB10090) shows detection of a band at ~81 KDa corresponding to mouse Ankrd26 protein.
Lane 1 Blank, Lane 2 MES cell lysate - 80 ug, Lane 3MES cell lysate-40 ug, Lane 4 293T-Ankrd26 transfected cell lysate-20 ug, Lane 5 control 293T celllysate-20 ug, Lane 6 BSA-Ankrd26 conjugate-20ng, Lane 7 BSA-500 ng, Lane 8 BSA-100 ng, Lane 9 BSA-20 ng.
Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used.
Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed withthe primary antibody diluted to 1:1,000 followed by detection using ALP conjugated Gt-a-Rabbit IgG diluted to 1:3,000.
Sizeestimation was made by comparison to prestained MW markers as indicated.
Personal Communication.Ira Pastan, NIH, CCR, Bethesda, MD.