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上海信裕生物科技有限公司
MYBBP1A抗体

MYBBP1A抗体

商家询价

产品名称： MYBBP1A抗体

英文名称： MYBBP1AAntibody

产品编号： XY14524-1-

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 MYBBP1A抗体

Applications
In-house applications ELISA, WB, IHC, IF, FC, IP     Application not tested? Please see our Guarantee.
Species specificity Human; other species not tested.
Images
Prev12NextView more validation data
  • 14524-1-AP;HeLa cellsHeLa cells were subjected to SDS PAGE followed by western blot with 14524-1-AP(MYBBP1A antibody) at dilution of 1:500
  • 14524-1-AP;human kidneyImmunohistochemical of paraffin-embedded human kidney using 14524-1-AP(MYBBP1A antibody) at dilution of 1:100 (under 40x lens)
  • 14524-1-AP;HepG2Immunofluorescent analysis of HepG2 cells, using MYBBP1A antibody 14524-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
  • 14524-1-AP;HEK-293 cellsIP Result of anti-MYBBP1A (IP:14524-1-AP, 4ug; Detection:14524-1-AP 1:500) with HEK-293 cells lysate 2800ug.
  • 14524-1-AP;HepG2 cells1X10^6 HepG2 cells were stained with 0.2ug MYBBP1A antibody (14524-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
Positive WB detected in HeLa cells, HEK-293 cells
Positive IP detected in HEK-293 cells
Positive IHC detected in Human kidney tissue
Positive IF detected in HepG2 cells
Positive FC detected in HepG2 cells
Recommended dilution WB : 1:500-1:5000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200
 MYBBP1A抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen MYBBP1A fusion protein ag6008 Full name MYB binding protein (P160) 1a
Calculated molecular
weight		 149 kDa Observed molecular 
weight		 160kd
GenBank accession 
number		 BC050546 Gene ID (NCBI) 10514
Gene symbol MYBBP1A Synonyms FLJ37886, MYB binding protein (P160) 1a, Myb binding protein 1A, MYBBP1A, P160, PAP2
Background MYBBP1A抗体

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996]. MYBBP1A抗体

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