Guanylin,鸟苷酸环化酶激活蛋白1抗体

产品编号HZ-11621R
英文名称Guanylin
中文名称鸟苷酸环化酶激活蛋白1抗体
别 名GapI; GCAP-I; Guanylate cyclase activating protein 1; Guanylate cyclase activator 2A (guanylin 2 intestinal heat-stable); Guanylate cyclase activator 2A; Guanylate cyclase-activating protein 1; Guanylate cyclase-activating protein I; Guanylin; GUC2A_HUMAN; GUCA 2; GUCA2; GUCA2A; STARA.
说 明 书0.1ml 0.2ml
研究领域神经生物学 生长因子和激素 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Pig, Cow, Horse, Sheep,
Guanylin,鸟苷酸环化酶激活蛋白1抗体产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量10kDa
细胞定位分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Guanylin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Guanylin,鸟苷酸环化酶激活蛋白1抗体PubMedPubMed
产品介绍background:
The family of guanylin regulatory peptides, including guanylin and uroguanylin, are strongly expressed in intestinal mucosa and regulate intestinal fluid secretion during digestion. Guanylins are also involved in acid neutralization and the regulation of membrane-bound guanylate cyclase signaling molecules. Guanylin and uroguanylin are secreted primarily in the stomach, intestine, and colon. Guanylin is also detected in plasma. Guanylin is an endogenous activator of intestinal guanylate cyclase. It stimulates intestinal guanylate cyclase through the same receptor binding region as the heat-stable enterotoxins. Gut enterochromaffin cells synthesize guanylin to be a prohormone of 115 amino acids which is then is processed to the molecular form of 94 amino acids. This 10kDa form is found circulating in the blood.

Function:
Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins.

Subcellular Location:
Secreted.

Tissue Specificity:
Highly expressed in ileum and colon. Found in plasma.

Similarity:
Belongs to the guanylin family.

Database links:
Entrez Gene: 2980 Human
Entrez Gene: 14915 Mouse
Entrez Gene: 25656 Rat
Omim: 139392 Human
SwissProt: Q02747 Human
SwissProt: P33680 Mouse
SwissProt: P28902 Rat
Unigene: 778 Human
Unigene: 2614 Mouse
Unigene: 11388 Rat

Guanylin,鸟苷酸环化酶激活蛋白1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Guanylin) Polyclonal Antibody, Unconjugated (HZ-11621R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.