FITC标记的装配衔接蛋白180抗体
产品名称： FITC标记的装配衔接蛋白180抗体
英文名称： Anti-AP180/FITC
产品编号： HZ-11333R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-AP180/FITC Conjugated antibody
FITC标记的装配衔接蛋白180抗体
| 产品编号 | bs-11333R-FITC |
| 英文名称 | Anti-AP180/FITC |
| 中文名称 | FITC标记的装配衔接蛋白180抗体 |
| 别 名 | AP 180; AP3; Assembly Protein 180 kDa; Assembly Protein AP180; CALM; Clathrin coat assembly protein; Clathrin coat assembly protein AP180; Clathrin coat associated protein AP180; DKFZp781O051; F120; KIAA0656; NP185; pp155; SNAP 91; SNAP91; 91kDa synaptosomal associated protein; Synaptosomal associated protein 91 kD homolog; Synaptosomal associated protein 91 kDa homolog; AP180_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 神经生物学 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 92kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AP180 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Clathrin-coated pits and vesicles are assembled for receptor-mediated endocytosis through interaction with Clathrin associated protein complexes. Vesicle transport is mediated from the trans-Golgi network by the adapter complex AP-1 and from the plasma membrane by the AP-2 complex. The AP-1 and AP-2 adapter protein complexes consist of Clathrin binding Adaptin proteins (g and b1 for AP-1, a and b2 for AP-2) and two smaller subunits known as AP50 and AP17. The a and b Adaptin chains have a similar two-domain organization with C-terminal domains that vary in both sequence and length. a-Adaptin splice variants A and C display variable relative expression levels and differential distribution in different tissues. AP-3 (also designated AP180 or F1-20) is a synapse-specific Clathrin assembly protein. The protein CALM (Clathrin assembly protein lymphoid myeloid leukemia) is highly homologous to AP180 and may also be involved in Clathrin assembly. Function: AP180 is a clathrin binding phospho-protein and faciliates the formation of clathrin coats. This protein is also known as pp155, NP185, F1-20, and AP-3. During neurotransmitter release synaptic vesicles fuse with the presynaptic plasma membrane. A whole protein machinery consisting of amphiphysin, clathrin, endophilin and synaptojanin is involved in the subsequent endocytotic recycling of the synaptic vesicles. Subunit: Binds AP2A2. Interacts with AP2B1; clathrin competes with SNAP91 (By similarity). Subcellular Location: Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane. Post-translational modifications: Thr-310 can be modified by the addition of N-acetylglucosamine which can be further phosphorylated. There is no evidence for direct Thr-310 phosphorylation (By similarity). Similarity: Contains 1 ENTH (epsin N-terminal homology) domain. Database links: Entrez Gene: 9892 Human Entrez Gene: 20616 Mouse Entrez Gene: 65178 Rat Omim: 607923 Human SwissProt: O60641 Human SwissProt: Q61548 Mouse SwissProt: Q05140 Rat Unigene: 368046 Human Unigene: 281651 Mouse Unigene: 11022 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
通过与网格蛋白相关蛋白复合物的相互作用，网格蛋白包被的凹坑和囊泡被组装成受体介导的内吞作用。囊泡运输是由转位复合物AP-1介导的跨高尔基网络介导的，而由AP-2复合物介导的质膜转运。AP-1和AP-2适配器蛋白复合物由网格蛋白结合的适应性蛋白（G和B1为AP-1，A和B2为AP-2）和两个较小的亚基称为AP50和AP17。A和B Adaptin链具有类似的两个域组织与C-端结构域在序列和长度上变化。A剪接变异体A和C在不同组织中显示可变的相对表达水平和差异分布。AP-3（也被命名为AP180或F1-20）是突触特异性网格蛋白组装蛋白。蛋白平静（网格蛋白装配蛋白淋巴样髓性白血病）与AP180高度同源，也可能参与网格蛋白组装。