NSMase2,中性鞘磷脂2抗体

产品编号HZ-11193R
英文名称NSMase2
中文名称中性鞘磷脂2抗体
别 名N-SMase2; Cca1; neutral sphingomyelinase 2; Confluent 3Y1 cell-associated protein 1; Neutral sphingomyelinase 2; Neutral sphingomyelinase II; NSMA2_HUMAN; nSMase-2; nSMase2; Smpd3; Sphingomyelin phosphodiesterase 3.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 脂蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
NSMase2,中性鞘磷脂2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量71kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human NSMase2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
NSMase2,中性鞘磷脂2抗体PubMedPubMed
产品介绍background:
N-SMase2 (neutral sphingomyelinase 2), also known as NSMASE2 or SMPD3 (sphingomyelin phosphodiesterase 3), is a ubiquitously expressed 655 amino acid member of the magnesium-dependent phosphohydrolase protein family. Localized to the membrane of the Golgi apparatus, N-SMase2 functions to catalyze the hydrolysis of sphingomyelin to form ceramide and phosphocholine—two proteins that mediate cell growth arrest and apoptosis. N-SMase2 is enzymatically activated by unsaturated fatty acids and phosphatidylserine and, through regulation of ceramide synthesis, is involved in growth suppression and postnatal development. Expression of N-SMase2 is upregulated during the G0/G1 phases of the cell cycle and optimal N-SMase2 activity occurs at a slightly basic pH of 7.5. N-SMase2 deficiency is the cause of chondrodysplasia, a genetic disorder characterized by impaired bone growth that leads to short stature, bowlegs and underdeveloped joints.

Function:
Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.

NSMase2,中性鞘磷脂2抗体Subunit:
Belongs to the neutral sphingomyelinase family.

Subcellular Location:
Golgi apparatus membrane. Cell membrane. May localize to detergent-resistant subdomains of Golgi membranes of hypothalamic neurosecretory neurons. According to PubMed:15051724, it localizes to plasma membrane in confluent contact-inhibited cells.

Tissue Specificity:
Predominantly expressed in brain.

Similarity:
Belongs to the neutral sphingomyelinase family.

Gene ID:
555112

Database links:
Entrez Gene: 55512 Human
Omim: 605777 Human
SwissProt: Q9NY59 Human
Unigene: 368421 Human

NSMase2,中性鞘磷脂2抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.