ERO1L,内质网氧化物蛋白Ero1-Lα抗体

产品编号HZ-10551M
英文名称ERO1L
中文名称内质网氧化物蛋白Ero1-Lα抗体
别 名Endoplasmic oxidoreductin 1 like protein; Endoplasmic oxidoreductin-1-like protein; ERO1 alpha; ERO1 L; ERO1 Lalpha; ERO1 like protein alpha; ERO1-L; ERO1-L-alpha; ERO1-like (S. cerevisiae); ERO1-like protein alpha; ERO1A; ERO1A_HUMAN; ERO1L; Oxidoreductin 1 Lalpha; Oxidoreductin-1-L-alpha.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 转运蛋白
抗体来源Mouse
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Rabbit,
ERO1L,内质网氧化物蛋白Ero1-Lα抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量52kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ERO1L
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ERO1L,内质网氧化物蛋白Ero1-Lα抗体PubMedPubMed
产品介绍background:
Ero1-La is an essential oxidoreductase that oxidizes proteins and is required for the folding of immunoglobulins. Ero-1La covalently binds with PDI (protein disulfide-isomerase) and together they produce disulfide bonds between proteins in the endoplasmic reticulum. Ero1-La and SIRT1 regulate adiponectin secretion from adipose tissue. Ero1-La and associated proteins also modulate PPARg (peroxisome proliferator-activated receptor g) and SIRT1 activities. Ero1-La is stimulated by hypoxia, suggesting that it is regulated through the HIF (hypoxia inducible transcrip-tion factor) pathway. Ero1-La is ubiquitously expressed at low levels but expressed at high levels in upper digestive tract and esophagus. Ero1-La may function both as a monomer and a homodimer.

Function:
Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.

Subunit:
Predominantly monomer. May function both as a monomer and a homodimer. Interacts with PDILT

ERO1L,内质网氧化物蛋白Ero1-Lα抗体Subcellular Location:
Endoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.

Tissue Specificity:
Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.

Post-translational modifications:
N-glycosylated.
The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center.
The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397.

DISEASE:

Similarity:
Belongs to the EROs family.

Gene ID:
30001

Database links:
Entrez Gene: 30001 Human
Entrez Gene: 50527 Mouse
Entrez Gene: 171562 Rat
Omim: 615435 Human
SwissProt: Q96HE7 Human
SwissProt: Q8R180 Mouse
SwissProt: Q8R4A1 Rat
Unigene: 592304 Human
Unigene: 387108 Mouse
Unigene: 64648 Rat

ERO1L,内质网氧化物蛋白Ero1-Lα抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.