EAP30,EAP30蛋白抗体

产品编号HZ-14482R
英文名称EAP30
中文名称EAP30蛋白抗体
别 名Dot3; ELL associated protein of 30 kDa; ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; hVps22; snf8; SNF8 ESCRTII complex subunit homolog (S cerevisiae); SNF8_HUMAN; Vacuolar sorting protein SNF8; Vacuolar-sorting protein SNF8; VPS22.
说 明 书0.1ml 0.2ml
研究领域转录调节因子 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep,
EAP30,EAP30蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量29kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human EAP30
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
EAP30,EAP30蛋白抗体PubMedPubMed
产品介绍background:
SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM, Mar 2008]

Function:
Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4.

EAP30,EAP30蛋白抗体Subcellular Location:
Cytoplasm. Endosome membrane. Nucleus. Late endosome membrane. Recruited to the endosome membrane to participate in vesicle formation.

Post-translational modifications:

Similarity:
Belongs to the SNF8 family.

Gene ID:
11267

Database links:
Entrez Gene: 11267 Human
Entrez Gene: 27681 Mouse
Entrez Gene: 287645 Rat
Entrez Gene: 492771 Zebrafish
Omim: 610904 Human
SwissProt: Q8IXY3 Human
SwissProt: Q96H20 Human
SwissProt: Q9CZ28 Mouse
SwissProt: Q5RK19 Rat
SwissProt: Q5U3V9 Zebrafish
Unigene: 127249 Human
Unigene: 372235 Mouse
Unigene: 162167 Rat
Unigene: 117433 Zebrafish
Unigene: 84729 Zebrafish

EAP30,EAP30蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.