AP180,装配衔接蛋白180抗体

产品编号HZ-11333R
英文名称AP180
中文名称装配衔接蛋白180抗体
别 名AP 180; AP3; Assembly Protein 180 kDa; Assembly Protein AP180; CALM; Clathrin coat assembly protein; Clathrin coat assembly protein AP180; Clathrin coat associated protein AP180; DKFZp781O051; F120; KIAA0656; NP185; pp155; SNAP 91; SNAP91; 91kDa synaptosomal associated protein; Synaptosomal associated protein 91 kD homolog; Synaptosomal associated protein 91 kDa homolog; AP180_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep,
AP180,装配衔接蛋白180抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量92kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human AP180
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
AP180,装配衔接蛋白180抗体PubMedPubMed
产品介绍background:
Clathrin-coated pits and vesicles are assembled for receptor-mediated endocytosis through interaction with Clathrin associated protein complexes. Vesicle transport is mediated from the trans-Golgi network by the adapter complex AP-1 and from the plasma membrane by the AP-2 complex. The AP-1 and AP-2 adapter protein complexes consist of Clathrin binding Adaptin proteins (g and b1 for AP-1, a and b2 for AP-2) and two smaller subunits known as AP50 and AP17. The a and b Adaptin chains have a similar two-domain organization with C-terminal domains that vary in both sequence and length. a-Adaptin splice variants A and C display variable relative expression levels and differential distribution in different tissues. AP-3 (also designated AP180 or F1-20) is a synapse-specific Clathrin assembly protein. The protein CALM (Clathrin assembly protein lymphoid myeloid leukemia) is highly homologous to AP180 and may also be involved in Clathrin assembly.

Function:
AP180 is a clathrin binding phospho-protein and faciliates the formation of clathrin coats. This protein is also known as pp155, NP185, F1-20, and AP-3. During neurotransmitter release synaptic vesicles fuse with the presynaptic plasma membrane. A whole protein machinery consisting of amphiphysin, clathrin, endophilin and synaptojanin is involved in the subsequent endocytotic recycling of the synaptic vesicles.

Subunit:
Binds AP2A2. Interacts with AP2B1; clathrin competes with SNAP91 (By similarity).

Subcellular Location:
Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.

Post-translational modifications:
Thr-310 can be modified by the addition of N-acetylglucosamine which can be further phosphorylated. There is no evidence for direct Thr-310 phosphorylation (By similarity).

Similarity:
Contains 1 ENTH (epsin N-terminal homology) domain.

AP180,装配衔接蛋白180抗体Database links:
Entrez Gene: 9892 Human
Entrez Gene: 20616 Mouse
Entrez Gene: 65178 Rat
Omim: 607923 Human
SwissProt: O60641 Human
SwissProt: Q61548 Mouse
SwissProt: Q05140 Rat
Unigene: 368046 Human
Unigene: 281651 Mouse
Unigene: 11022 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-AP180 Polyclonal Antibody, Unconjugated(HZ-11333R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining