该细胞系是1972年由GiardDJ通过肺癌组织移植培养建系的，源自一位58岁的白人男性。A549能通过胞苷二磷脂酰胆碱途径合成富含不饱和脂肪酸的卵磷脂；角蛋白阳性。

细胞名称：人非小细胞肺癌细胞

细胞简称：A549

产品货号：TCH-C116

种属来源：人

组织来源：肺

疾病特征：肺癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：D/F12+10%FBS+1%P/S

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

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