人多能干细胞定量PCR芯片试剂盒
产品名称： 人多能干细胞定量PCR芯片试剂盒
英文名称： GQH-hPSC
产品编号： GK002
产品价格： 0
产品产地： 美国
品牌商标： sciencell
更新时间： 2024-11-06T10:04:08
使用范围： null
- 联系人 : 胡小姐
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人多能干细胞定量PCR芯片试剂盒描述：
ScienCells GeneQuery™ human pluripotent stem cell qPCR array kit (GQH-hPSC) is designed to facilitate gene expression profiling of key genes involved in maintaining pluripotency and self-renewal of hPSC, and genes regulated during differentiation of hPSC. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Pluripotent Stem Cell qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ACTC1 |
CDX2 |
EN2 |
FOXA2 |
GFAP |
ISL1 |
MIXL1 |
NOG |
PECAM1 |
SOX1 |
TBX6 |
β-actin |
|
B |
AFP |
CER1 |
ENO2 |
FOXD3 |
GJA1 |
KLF4 |
MYF5 |
NPM1 |
POU5F1 |
SOX2 |
TCF2 |
GAPDH |
|
C |
ALB |
COL1A1 |
EOMES |
FOXJ3 |
GSC |
LEFTYA |
MYOD1 |
NR5A2 |
PTF1A |
SOX7 |
TCF3 |
LDHA |
|
D |
ALDH1A1 |
COL2A1 |
ESG1 |
GATA2 |
HAND1 |
LEFTYB |
NANOG |
OLIG2 |
REST |
SOX17 |
TDGF1 |
NONO |
|
E |
BRIX1 |
CXCR4 |
ESRRB |
GATA4 |
HDAC2 |
LIN28 |
NEUROD |
OTX2 |
REX1 |
T |
TERT |
PPIH |
|
F |
CD34 |
DES |
FGF4 |
GATA6 |
HLXB9 |
MEF2C |
NCAM1 |
PAX2 |
RUNX1 |
TAT |
TUBB3 |
GDC |
|
G |
CD90 |
DNMT3B |
FGF5 |
GBX2 |
HNF4A |
MEIS1 |
NES |
PAX6 |
RUNX2 |
TBX3 |
UTF1 |
PPC |
|
H |
CDH1 |
EMX2 |
FLT1 |
GDF3 |
IDH1 |
MESP1 |
NKX2.5 |
PDX-1 |
SALL4 |
TBX5 |
VIM |
NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK002 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-hPSC |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | For research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |