分子量:109 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Contactin-2, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been precoated with 50 μl/well of the rhContactin2 solution at 8-30 μg/mL.

产品特性:Human Contactin-2, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 109 kDa. The predicted N-terminus is Ser 31. The reducing (R) protein migrates as 140 kDa in SDS-PAGE  due to glycosylation.

产品背景:Contactin-2 is also known as CNTN2, TAX1 (transiently-expressed axonal glycoprotein), TAG1 (transient axonal glycoprotein), and axonin-1, and is a member of the immunoglobulin superfamily. CNTN2 consists of six Ig-like domains and four fibronectin type III domains, and is anchored to the membrane by glycosylphosphatidylinositol (GPI), whereas the soluble form can be released by a GPI-specific phospholipase. As a neural cell adhesion molecule expressed by a subset of neuronal populations in the developing CNS and PNS, CNTN2 mediates cell-cell interactions either via homophilic, or heterophilic contacts with various adhesion molecules including NgCAM, NrCAM, NCAM and neurocan. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. It may also be involved in glial tumorigenesis and may provide a potential target for therapeutic intervention.