Anti-NeuN抗体[EPR12763] - Neuronal Marker-抗体-抗体-生物在线
深圳市宇德立生物科技有限公司
Anti-NeuN抗体[EPR12763] - Neuronal Marker

Anti-NeuN抗体[EPR12763] - Neuronal Marker

商家询价

产品名称： Anti-NeuN抗体[EPR12763] - Neuronal Marker

英文名称： FLJ56884 antibody FLJ58356 antibody Fox-1 homolog C antibody fox1 homolog C antibody Fox3 antibody FOX3NeuN antibody hexaribonucleotide binding protei

产品编号： ab177487

产品价格： null

产品产地： 英国

品牌商标： abcam

更新时间： 2023-09-21T08:08:01

使用范围：

深圳市宇德立生物科技有限公司
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进入展台

 

  • 经测试应用IHC-FoFr, Flow Cyt, IHC-Fr, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Goat, Cat, Dog, Human, Zebrafish, Marmoset (common)
  • 免疫原

    Synthetic peptide within Human NeuN aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: A6NFN3

    Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Cerebellum; Rat cerebellum; Human Fetal brain as well as the following whole cell lysates: 293T, HeLa and SH-SY5Y lysates; SH-SY-5Y cells. Flow Cyt: U-87MG cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-NeuN antibody (Alexa Fluor® 488) [EPR12763] - Neuronal Marker (ab190195)

    Anti-NeuN antibody (Alexa Fluor® 647) [EPR12763] - Neuronal Marker (ab190565)

性能

应用

Our Abpromise guarantee covers the use of ab177487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Abreviews 说明
IHC-FoFr   1/500 - 1/6000.
Flow Cyt   1/100.
IHC-Fr   1/500.
IHC-P   1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/800.
WB   1/1000 - 1/10000. Detects a band of approximately 48,50 kDa (predicted molecular weight: 34 kDa).

For unpurified use at 1/1000 - 1/2000.
ICC/IF   1/300.

For unpurified use at 1/80.

靶标

  • 功能RNA-binding protein that regulates alternative splicing events.
  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
  • 细胞定位Nucleus. Cytoplasm.

  • Anti-NeuN antibody [EPR12763] - Neuronal Marker 图像

    • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
      Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

      ​An independent comparison of commercially available NeuN clones in IHC-Fr (acetone-fixed mouse dentate gyrus sections)

       

      Competitor A: Leading mouse monoclonal

      Competitor B: Non-Abcam rabbit monoclonal

       

       

      ab177487 produces intense, specific staining with minimal background, even at half the dilution of competing antibodies.

    • TUNEology 波长可调检测卡盒-->