• 特异性No cross reactivity with other proteins.
• 经测试应用IHC-P, WB, ICCmore details
• 种属反应性
  与反应: Human
  预测可用于: Mouse, Rat, Sheep, Cow
• 免疫原

  Synthetic peptide corresponding to Human 14-3-3 sigma aa 140-156 (internal sequence).
  Sequence:

  KKRIIDSARSAYQEAMD

  
  Database link: P31947

   

  Run BLAST with Run BLAST with 
• 阳性对照
  • Human tonsil and oesophagus squama cancer tissue. HeLa, A549, COLO320 amd SE620 cell lysates.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
  Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
• 浓度100 µg 浓度为 0.5 mg/ml 
  
• 纯度Immunogen affinity purified
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Neurology process
   
  • Neural Signal Transduction

  • Signal Transduction
   
  • Adapters
   
  • Cytoplasmic

  • Neuroscience
   
  • Cell Type Marker
   
  • Neuron marker
   
  • Synapse marker

  • Stem Cells
   
  • Signaling Pathways
   
  • Wnt
   
  • 14-3-3

应用

靶标

• 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
  p53-regulated inhibitor of G2/M progression.
• 组织特异性Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
• 序列相似性Belongs to the 14-3-3 family.
• 翻译后修饰Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
• 细胞定位Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
• Information by UniProt
• 数据库链接
  • Entrez Gene: 528453 Cow
  • Entrez Gene: 2810 Human
  • Entrez Gene: 55948 Mouse
  • Entrez Gene: 313017 Rat
  • Entrez Gene: 443032 Sheep
  • Omim: 601290 Human
  • SwissProt: Q0VC36 Cow
  • SwissProt: P31947 Human

  see all
• 别名
  • 14 3 3 protein sigma antibody
  • 14-3-3 protein sigma antibody
  • 1433S_HUMAN antibody

  see all

Anti-14-3-3 sigma antibody 图像

• 

  Western blot - Anti-14-3-3 sigma antibody (ab190960)
  All lanes : Anti-14-3-3 sigma antibody (ab190960) at 0.5 µg/ml
  
  Lane 1 : HeLa cell lysate
  Lane 2 : A549 cell lysate
  Lane 3 : A549 cell lysate
  Lane 4 : COLO320 cell lysate
  Lane 5 : SE620 cell lysate
  
  
  Predicted band size : 27 kDa
  
• 

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