phospho-CTNNBIP1 (Ser36),磷酸化β链接素相互作用蛋白1抗体

产品编号HZ-5265R
英文名称phospho-CTNNBIP1 (Ser36)
中文名称磷酸化β链接素相互作用蛋白1抗体
别 名Beta catenin interacting protein 1; Beta catenin interacting protein ICAT; Catenin beta interacting protein 1; CTNNBIP 1; ICAT; Inhibitor of beta catenin and Tcf 4; MGC15093; CNBP1_HUMAN.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域细胞生物 免疫学 结合蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Cow, Rabbit,
phospho-CTNNBIP1 (Ser36),磷酸化β链接素相互作用蛋白1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量9kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human CTNNBIP1 around the phosphorylation site of Ser36
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-CTNNBIP1 (Ser36),磷酸化β链接素相互作用蛋白1抗体PubMedPubMed
产品介绍background:
CTNNBIP1 (Catenin, beta interacting protein 1) binds to beta-catenin and prevents the interaction between beta-catenin and TCF transcription factor family members. It is a negative regulator of the Wnt signaling pathway.

Function:
Prevents the interaction between CTNNB1 and TCF family members, and acts as negative regulator of the Wnt signaling pathway.

Subunit:
Binds CTNNB1.

Subcellular Location:
Cytoplasm (By similarity). Nucleus (By similarity).

Similarity:
Belongs to the CTNNBIP1 family.

phospho-CTNNBIP1 (Ser36),磷酸化β链接素相互作用蛋白1抗体Database links:
UniProtKB/Swiss-Prot: Q9NSA3.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human bladder carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-phospho-CTNNBIP1 (Ser36) Polyclonal Antibody, Unconjugated(HZ-5265R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining