β-胡萝卜素-15,15-单加氧酶1(βCMO1)重组蛋白

YB246Hu013
Recombinant Beta-Carotene-15,15"-Monooxygenase 1 (bCMO1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
 β-胡萝卜素-15,15-单加氧酶1(βCMO1)重组蛋白Source: Prokaryotic expression. Host: E. coli
Residues: Gly6~Pro206
Tags: N-terminal His-Tag
Subcellular Location: Cytosol. Purity: >99%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.9
 β-胡萝卜素-15,15-单加氧酶1(βCMO1)重组蛋白Predicted Molecular Mass: 26.6kDa
Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
 β-胡萝卜素-15,15-单加氧酶1(βCMO1)重组蛋白protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE