90kDa热休克蛋白αB1(HSP90αB1)重组蛋白

YB522Ra011
Recombinant Heat Shock Protein 90kDa Alpha B1 (HSP90aB1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
90kDa热休克蛋白αB1(HSP90αB1)重组蛋白[ PROPERTIES ]
Residues: Met1~Asp724
Tags: N-terminal His-Tag
Accession: P08238
Host: E. coli
Subcellular Location: Cytoplasm. Melanosome.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
90kDa热休克蛋白αB1(HSP90αB1)重组蛋白sarcosyl and preservative.
Predicted isoelectric point: 5.1
Predicted Molecular Mass: 85.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MPEEVHHGEE EVETFAFQAE IAQLMSLIIN TFYSNKEIFL RELISNASDA LDKIRYESLT
DPSKLDSGKE LKIDIIPNPQ ERTLTLVDTG IGMTKADLIN NLGTIAKSGT KAFMEALQAG
ADISMIGQFG VGFYSAYLVA EKVVVITKHN DDEQYAWESS AGGSFTVRAD HGEPIGRGTK
VILHLKEDQT EYLEERRVKE VVKKHSQFIG YPITLYLEKE REKEISDDEA EEEKGEKEEE
90kDa热休克蛋白αB1(HSP90αB1)重组蛋白D K D D E E K P K I E D V G S D E E D D S G K D K K K K T K KI K E K Y I D Q E E L N K T K P IW T
RNPDDITQEEYGEFYKSLTN DWEDHLAVKH FSVEGQLEFR ALLFIPRRAP FDLFENKKKK
NNIKLYVRRV FIMDSCDELI PEYLNFIRGV VDSEDLPLNI SREMLQQSKI LKVIRKNIVK
KCLELFSELA EDKENYKKFY EAFSKNLKLG IHEDSTNRRR LSELLRYHTS QSGDEMTSLS
EYVSRMKETQ KSIYYITGES KEQVANSAFV ERVRKRGFEV VYMTEPIDEY CVQQLKEFDG
KSLVSVTKEG LELPEDEEEK KKMEESKAKF ENLCKLMKEI LDKKVEKVTI SNRLVSSPCC
IVTSTYGWTA NMERIMKAQA LRDNSTMGYM MAKKHLEINP DHPIVETLRQ KAEADKNDKA
VKDLVVLLFE TALLSSGFSL EDPQTHSNRI YRMIKLGLGI DEDEVAAEEP NAAVPDEIPP
LEGDEDASRM EEVD
[ REFERENCES ]
1. Rebbe N.F., et al. (1987) Gene 53:235-245.
2. Rebbe N.F., et al. (1989) J. Biol. Chem. 264:15006-15011.
3. Hoffmann T., Hovemann B. (1988) Gene 74:491-501.
4. Lees-Miller S.P., Anderson C.W. (1989) J. Biol. Chem. 264:2431-2437.