Eph receptor A1,酪氨酸蛋白激酶受体A1抗体

产品编号HZ-7498R
英文名称Eph receptor A1
中文名称酪氨酸蛋白激酶受体A1抗体
别 名EPH; Eph tyrosine kinase 1; EphA1; Ephrin receptor Eph A1; Ephrin type A receptor 1; EPHT 1; EPHT; Erythropoietin producing hepatoma amplified sequence; Esk; EPHA1_HUMAN; MGC163163; Oncogene EPH; Soluble EPHA1 variant 1; Soluble EPHA1 variant 2; Tyrosine protein kinase receptor EPH.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 神经生物学 信号转导 通道蛋白 细胞膜受体
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep,
Eph receptor A1,酪氨酸蛋白激酶受体A1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量105kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Eph receptor A1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Eph receptor A1,酪氨酸蛋白激酶受体A1抗体PubMedPubMed
产品介绍background:
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]

Function:
The Eph family of receptors comprises the largest known family of receptor tyrosine kinases. Ligands of Eph family receptors are structurally related membrane-bound proteins that can be subdivided into two major subclasses, ephrin-A and ephrin-B. Expression of Eph receptors is tissue specific and appears to be tied to developmental events. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.

Eph receptor A1,酪氨酸蛋白激酶受体A1抗体Subunit:
Homodimer. Forms a signaling complex with LCK; PTK2B/PYK2 and PI3-kinase upon activation by EFNA1; regulates T-lymphocytes migration. Interacts (via SAM domain) with ILK (via ANK repeats); stimulated by EFNA1 but independent of the kinase activity of EPHA1. Interacts (kinase activity-dependent) with PTK2/FAK1.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein

Post-translational modifications:
Phosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.
Ubiquitinated.

Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 1 Eph LBD (Eph ligand-binding) domain.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.

Gene ID:
2041

Database links:
Entrez Gene: 2041 Human
Entrez Gene: 13835 Mouse
Entrez Gene: 312279 Rat
Omim: 179610 Human
SwissProt: P21709 Human
SwissProt: Q60750 Mouse
Unigene: 89839 Human
Unigene: 133330 Mouse
Unigene: 218221 Rat

Eph receptor A1,酪氨酸蛋白激酶受体A1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Cell: Mouse Splenocyte (Ice 70% ethanol for the night). Concentration: 1:100(primary antibody) Incubation: 40 minutes.  Host/Blank:Mouse Splenocyte.  Flow cytometric analysis of Rabbit Anti-Eph receptor A1/PE Conjugated antibody (HZ-7498R-PE) (green) compared with control in the absence of primary antibody (blue) followed by mouse splenocyte.