肌球蛋白重链10(MYH10)重组蛋白

YB423Hu012
Recombinant Myosin Heavy Chain 10, Non Muscle (MYH10)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 肌球蛋白重链10(MYH10)重组蛋白[ PROPERTIES ]
Residues: Thr438~Lys716 (Accession # P35580),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Cell projection,
lamellipodium.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
 肌球蛋白重链10(MYH10)重组蛋白Predicted isoelectric point: 6.3
Predicted Molecular Mass: 36.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
 肌球蛋白重链10(MYH10)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSG LVP RGSH MASM TGGQQM GRGSEF - TYE RL FRWLV HRI
NKALDRTKRQ GASFIGILDI AGFEIFELNS FEQLCINYTN EKLQQLFNHT MFILEQEEYQ
REGIEWNFID FGLDLQPCID LIERPANPPG VLALLDEECW FPKATDKTFV EKLVQEQGSH
SKFQKPRQLK DKADFCIIHY AGKVDYKADE WLMKNMDPLN DNVATLLHQS SDRFVAELWK
DEIQNIQRAS FYDSVSGLHE PPVDRIVGLD QVTGMTETAF GSAYKTKKGM FRTVGQLYKE
SLTKLMATLR NTNPNFVRCI IPNHEK