Cell Free DNA Isolation Protocol：

整个实验流程可在大约30min 内完成，包括蛋白酶解、DNA结合、快速洗涤、cfDNA 洗脱。

KIT CONTENTS

• cfDNA DNA Proteinase K

• Urine cfDNA Binding Solution

• cfDNA Magnetic beads

• cfDNA Wash Solution 1

• cfDNA Wash Solution 2

• cfDNA Elution Solution

• Control PCR Primers

REQUIRED MATERIALS NOT PROVIDED

• Ethanol, 100%

• Vortex mixer

• Heat block at 55°C

• Magnetic stands to hold 2 mL microcentrifuge tubes and 15 mL or 50 mL tubes

• Microcentrifuge or low-speed “picofuge”

• Nuclease-free 2 mL (1.5 mL and 0.5 mL optional) microcentrifuge tubes and 15 mL tubes

• Pipettors (P-1000, P-200, P-20) and tips

• Disposable pipettes (variable sizes depending on sample volume processed)

Selected Citations that Reference the Use of the NextPrep-Mag Urine cfDNA Isolation kit:

Streleckiene, G., Reid, H. M., Arnold, N., Bauerschlag, D., & Forster, M. (2018). Quantifying cell free DNA in urine: Comparison between commercial kits, impact of gender and inter-individual variation. BioTechniques,64(5), 225-230. doi:10.2144/btn-2018-0003