RFC4 monoclonal antibody (M01), clone 1C12
产品名称： RFC4 monoclonal antibody (M01), clone 1C12
英文名称： RFC4 monoclonal antibody (M01), clone 1C12
产品编号： H00005984-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant RFC4.
- Immunogen:
- RFC4 (AAH17452, 254 a.a. ~ 363 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- GKEITEKVITDIAGVIPAEKIDGVFAACQSGSFDKLEAVVKDLIDEGHAATQLVNQLHDVVVENNLSDKQKSIITEKLAEVDKCLADGADEHLQLISLCATVMQQLSQNC
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Cell lysate)
- RFC4 monoclonal antibody (M01), clone 1C12 Western Blot analysis of RFC4 expression in K-562 ( Cat # L009V1 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.
Lane 1: RFC4 transfected lysate(39.7 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to RFC4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged RFC4 is approximately 0.3ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of RFC4 over-expressed 293 cell line, cotransfected with RFC4 Validated Chimera RNAi ( Cat # H00005984-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RFC4 monoclonal antibody (M01), clone 1C12 (Cat # H00005984-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Application Image
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Transfected lysate)
- enlarge
- Western Blot (Recombinant protein)
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Sandwich ELISA (Recombinant protein)
- enlarge
- ELISA
- RNAi Knockdown (Antibody validated)
- enlarge
- Entrez GeneID:
- 5984
- GeneBank Accession#:
- BC017452
- Protein Accession#:
- AAH17452
- Gene Name:
- RFC4
- Gene Alias:
- A1,MGC27291,RFC37
- Gene Description:
- replication factor C (activator 1) 4, 37kDa
- Omim ID:
- 102577
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq
- Other Designations:
- A1 37 kDa subunit,RFC 37 kDa subunit,activator 1 37 kDa subunit,replication factor C (activator 1) 4 (37kD),replication factor C 4