Synonym: DMSO, Methyl sulfoxide 
CAS Number: 67-68-5 
Linear Formula: (CH3)2SO 
Molecular Weight: 78.13 
Beilstein Registry Number: 506008 
EC Number: 200-664-3 
MDL number: MFCD00002089 
PubChem Substance ID: 24893703 
Description
Frequently Asked Questions Live Chat and Frequently Asked Questions are available for this Product. 
Application DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS). 
Caution Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product. 
Other Notes This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels. 
Packaging 5 mL and 10 mL in flame sealed ampules, 100 mL in amber bottle 
Legal Information Hybri-Max is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. 
Protocols & Applications Protocol for cyropreservation 



性质
grade Hybri-Max 
vapor density 2.7 (vs air) 
vapor pressure 0.42 mmHg ( 20 °C) 
sterility sterile-filtered 
product line BioReagent 
assay ≥99.7% 
autoignition temp. 573 °F 
expl. lim. 42 %± , 63 °F 
total impurities endotoxin, tested 
refractive index n20/D 1.479(lit.) 
bp 189 °C(lit.) 
mp 16-19 °C(lit.) 
density 1.10 g/mL(lit.) 
suitability suitable for hybridoma 



安全性
Personal Protective Equipment Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter 
WGK Germany 1 
RTECS PV6210000 
Flash Point(F) 188.6 °F 
Flash Point(C) 87 °C 

参考文献
reference Pegg DE., Principles of cryopreservation Methods Mol. Biol. 368, 39-57, (2007) Abstract 
  Kleman MI, et al., Optimal conditions for freezing CHO-S and HEK293-EBNA cell lines: influence of Me2SO, freeze density, and PEI-mediated transfection on revitalization and growth of cells, and expression of recombinant protein Biotechnol. Bioeng. 100, 911-922, (2008) Abstract 
  Ling WL, Deng L, Lepore J, Cutler C, et. al., Improvement of monoclonal antibody production in hybridoma cells by dimethyl sulfoxide. Biotechnol. Prog. 19, 158-162, (2003) 
  Paynter SJ, Fuller BJ., Cryopreservation of mammalian oocytes. Methods Mol. Biol. 368, 313-324, (2007) 
Merck Merck 14,3259 
Beilstein Beil. 1,IV,1277 
Fieser Fieser 1,296 / Fieser 2,157 / Fieser 3,119 / Fieser 4,192 / Fieser 5,263 / Fieser 6,225 / Fieser 7,133 / Fieser 8,198 / Fieser 9,184 / Fieser 9,189 / Fieser 10,166 / Fieser 11,214 / Fieser 12,212 / Fieser 13,124 / Fieser 16,149 
  Aldrich MSDS 1, 1308:D / Corp MSDS 1 (2), 2440:C / FT-IR 2 (1), 1492:B / FT-IR 1 (1), 879:A / FT-NMR 1 (1), 1417:A / IR-Spectra (3), 529:A / NMR-Reference 2 (2), 781:A / RegBook 1 (1), 1043:A / Sax 6, 1201 / Structure Index 1, 162:A:1 / Vapor Phase 3, 831:A 

 打印预览
 

Product Reviews Review This Product
Overall: (5 out of 5) 
Kazzy  (read my reviews)
Location: OHIO
Research Experience: 16 or more
Purchasing Frequency: Monthly
Education: Ph.D.
Primary Field: Life ScienceGreat format we use the 10ml vialsApril 13, 2010 
Application: cell culture
"We buy the 10ml vials and make up one batch of freezing media. we then aliquat it into 5ml samples and freeze it- then when you need to freeze cells you only defrost as much as you need it keeps everything as sterile as possible- especially if you have new people"
Was this review helpful to you? Yes Yes No No (Report Inappropriate Review) (Report Inappropriate Review) 
Thank you! You have successfully submitted a comment for this review.
Post Comment Comment