重组人PD-L1蛋白（Recombinant Human PD-L1 Protein），GMP级

产品货号：GMP-TL727

产品描述（Description）

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

分子别名（Synonym）

CD274、B7-H1、PDCD1L1、PDCD1LG1

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列编码人PD-L1（NCBI Reference Sequence:NP_054862.1）表达带有His标签在C末端

分子量（Molecular Weight）

重组人PD-L1蛋白 包含222个氨基酸，预测理论分子量为26.9 kD

纯度（Purity）

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定 PD-L1(His 标签)1ug/mL(100 uL/孔)能够结合抗人PD-L1抗体的线性范围是0.5-5 ng/mL

稳定性（Stability）

冻干制剂可在 4℃保存 24 个月， 溶解后的液体可置于-20℃保存 6-12 个月， 避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1. DNA double-strand break repair pathway regulates PD-L1 expression in cancer cells Hiro Sato, Atsuko Niimi, Takaaki Yasuhara, Tiara Bunga Mayang Permata, Yoshihiko Hagiwara, Mayu Isono, Endang Nuryadi, Ryota Sekine, Takahiro Oike, Sangeeta Kakoti, Yuya Yoshimoto, Kathryn D. Held, Yoshiyuki Suzuki, Koji Kono, Kiyoshi Miyagawa, Takashi Nakano, Atsushi Shibata Nat Commun. 2017; 8: 1751. Published online 2017 Nov 24. doi: 10.1038/s41467-017-01883-9

  注意：For research and manufacturing use.