AEG-1/MTDH-Specific抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
AEG-1/MTDH-Specific抗体

AEG-1/MTDH-Specific抗体

商家询价

产品名称： AEG-1/MTDH-Specific抗体

英文名称： AEG-1/MTDH-SpecificAntibody

产品编号： XY13860-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 AEG-1/MTDH-SPECIFIC抗体

Applications
In-house applications ELISA, WB, IHC, IF, IP     Application not tested? Please see our Guarantee.
Published applications IHC, WB Publications 17 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human
Images
Prev123NextView more validation data
  • 13860-1-AP;MCF7 cellsMCF7 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP(AEG-1 antibody) at dilution of 1:1000
  • 13860-1-AP;A549 cellsWB result of AEG-1/MTDH antibody (13860-1-AP, 1:2000) with si-Control and si-MTDH transfected A549 cells.
  • 13860-1-AP;human breast cancer tissueImmunohistochemical of paraffin-embedded human breast cancer using 13860-1-AP(AEG-1 antibody) at dilution of 1:50 (under 10x lens)
  • 13860-1-AP;human breast cancer tissueImmunohistochemical of paraffin-embedded human breast cancer using 13860-1-AP(AEG-1 antibody) at dilution of 1:50 (under 40x lens)
  • 13860-1-AP;human prostate cancer tissueImmunohistochemical of paraffin-embedded human prostate cancer using 13860-1-AP(AEG-1 antibody) at dilution of 1:100 (under 10x lens)
  • 13860-1-AP;human prostate cancer tissueImmunohistochemical of paraffin-embedded human prostate cancer using 13860-1-AP(AEG-1 antibody) at dilution of 1:100 (under 40x lens)
  • 13860-1-AP;MCF-7 cellsImmunofluorescent analysis of MCF-7 cells, using MTDH antibody 13860-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
  • 13860-1-AP;MCF-7 cellsIP Result of anti-AEG-1 (IP:13860-1-AP, 3ug; Detection:13860-1-AP 1:1000) with MCF-7 cells lysate 3250ug.
Positive WB detected in MCF7 cells, A375 cells, A549 cells, DU 145 cells, HeLa cells, HepG2 cells, PC-3 cells, RAW 264.7 cells
Positive IP detected in MCF-7 cells
Positive IHC detected in Human prostate cancer tissue, human breast cancer tissue, human liver cancer tissue, human stomach cancer tissue, mouse brain tissue
Positive IF detected in MCF-7 cells
Recommended dilution WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:20-1:200
 AEG-1/MTDH-SPECIFIC抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen AEG-1/MTDH-Specific fusion protein ag4840 Full name Metadherin
Calculated molecular
weight		 582aa,64 kDa Observed molecular 
weight		 65-70 kDa
GenBank accession 
number		 BC045642 Gene ID (NCBI) 92140
Gene symbol MTDH Synonyms 3D3, 3D3/LYRIC, AEG 1, AEG1, LYRIC, LYRIC/3D3, metadherin, Metastasis adhesion protein, MTDH, Protein LYRIC
Background AEG-1/MTDH-SPECIFIC抗体

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH. AEG-1/MTDH-SPECIFIC抗体

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