FITC标记的周期素E抗体
产品名称： FITC标记的周期素E抗体
英文名称： Anti-Cyclin E1/FITC
产品编号： HZ-0573R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： Flow-Cyt=1:50-200 IF=1:50-200
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Rabbit Anti-Cyclin E1/FITC Conjugated antibody
FITC标记的周期素E抗体
| 英文名称 | Anti-Cyclin E1/FITC |
| 中文名称 | FITC标记的周期素E抗体 |
| 别 名 | CCNE 1; CCNE; CCNE1; Cyclin E1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1; CCNE1_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, |
| 产品应用 | Flow-Cyt=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 45kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat Cyclin E |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. Transcript Variant: This variant (1) contains a different 5' end region, which includes an upstream in-frame translation start codon, when compared to variant 2. The encoded protein has a 15 aa longer N-terminus, as compared to isoform 2. Subunit: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation. Subcellular Location: Nucleus. Tissue Specificity: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. Post-translational modifications: Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the cyclin family. Cyclin E subfamily. Database links: Entrez Gene: 898 Human Entrez Gene: 12447 Mouse Entrez Gene: 25729 Rat Omim: 123837 Human SwissProt: P24864 Human SwissProt: Q61457 Mouse SwissProt: P39949 Rat Unigene: 244723 Human Unigene: 16110 Mouse Unigene: 15455 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 细胞周期素E是调控细胞G-1→S期转变的关键因素。由于在多种肿瘤中的不适当表达,细胞周期素E现在已明确为原癌基因。 |
该基因编码的蛋白属于高度保守的cyclin家族，其成员特征是细胞周期中蛋白丰度的显著周期性。细胞周期蛋白是CDK激酶的调节因子。不同的周期蛋白表现出不同的表达和降解模式，这有助于每个有丝分裂事件的时间协调。该细胞周期蛋白与CDK2形成复合物，作为CDK2的调节亚基发挥作用，其活性是细胞周期G1/S转变所必需的。这种蛋白质聚集在G1-S相界，并随着细胞通过S期而降解。该基因在许多肿瘤中过度表达，导致染色体不稳定，从而可能参与肿瘤的发生。发现该蛋白与NPAT蛋白（核蛋白映射到ATM位点）的磷酸化相关，并且参与NPAT蛋白的磷酸化，该蛋白参与细胞周期调节组蛋白基因的表达，并且在没有pRB的情况下在促进细胞周期进展中发挥关键作用。已经描述了该基因的两个交替剪接转录变体，其编码不同的异构体。报道了两个附加剪接变异体，但尚未获得详细的核苷酸序列信息。转录变体：与变体2相比，这个变体（1）包含一个不同的5’末端区域，它包括一个帧内上游翻译起始密码子。与亚型2相比，编码的蛋白质具有更长的N-末端15 aa。