FITC标记的去整合素样金属蛋白酶2抗体
产品名称： FITC标记的去整合素样金属蛋白酶2抗体
英文名称： Anti-ADAM2/FITC
产品编号： HZ-5851R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-ADAM2/FITC Conjugated antibody
FITC标记的去整合素样金属蛋白酶2抗体
| 英文名称 | Anti-ADAM2/FITC |
| 中文名称 | FITC标记的去整合素样金属蛋白酶2抗体 |
| 别 名 | A disintegrin and metalloproteinase domain 2; ADAM 2; ADAM metallopeptidase domain 2; Adam2; ADAM2_HUMAN; AI323749; Cancer/testis antigen 15; CRYN1; CRYN2; CT15; Disintegrin and metalloproteinase domain-containing protein 2; Fertilin beta; Fertilin subunit beta; Ftnb; PH 30; PH 30b; PH-30; PH30; Ph30 beta; PH30-beta. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 激酶和磷酸酶 细胞骨架 细胞外基质 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 63kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADAM2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: ADAM-2, also known as PH-30?and Fertilin-? was first described as a sperm-egg fusion protein from guinea pig. A member of the metalloproteinase family containing disintigrin-like domains (ADAMs), the function of ADAM-2 is still poorly understood. Unlike ADAM-1, ADAM-2 does not contain the canonical HExxHxxxxxH zinc metalloproteinase motif, and is not thought to be proteolytically active. Like the other ADAMs, ADAM-2 domain structure consists of a signal sequence followed by a propeptide domain, a metalloproteinase domain, a disintegrin domain cysteine-rich domain, an EGF-like domain, a transmembrane domain, and a cytoplasmic domain. Three isoforms of ADAM-2 are reported to date, which differ in the beginning of the metalloproteinase domain and cysteine-rich domain. The sequences are coded from intronless genes. The longest ADAM-2 message encodes a protein of 735 amino acids, with a predicted mass of 82.5 kDa and a pI of 5.73. The 716 amino acid form of ADAM-2 has a deletion in the start of the metalloproteinase domain, relative to the longer form, and has a predicted mass of 80.2 kDa and pI of 5.75. The shorter form of 579 amino acids shared the deletion in the MP domain, and also has a deletion in the cystein-rich domain. Localized on the surface of sperm, the ADAM-2 isoforms are thought to form a heterodimer with ADAM-1 (fertilin-a), and facilitate sperm-egg fusion, although there is some controversy about the precise actions the proteins play. Integrin a6? on the egg surface is thought to dock with a QDECD motif in the disintegrin domain of ADAM-2, and there is some speculation that ADAM-1/ADAM-2 heterodimewr initiated ADAM-3 production on the cell surface. Function: Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein. Subunit: Heterodimer with ADAM1/fertilin subunit alpha. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed specifically in testis. Post-translational modifications: The prodomain and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa. Similarity: Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain. Database links: Entrez Gene: 2515 Human Omim: 601533 Human SwissProt: Q99965 Human Unigene: 177959 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 细胞外基质蛋白 |
ADAM-2，又称PH-30？施肥呢？首次被描述为豚鼠精子卵融合蛋白。金属蛋白酶家族成员中含有DytGrin样结构域（亚当斯），ADAM-2的功能至今仍不清楚。与ADAM-1不同的是，ADAM-2不包含典型的HxXHXXXXH锌金属蛋白酶基序，并且不被认为是蛋白水解活性的。与其他亚当斯一样，ADAM-2结构域由一个信号序列组成，随后是一个前肽结构域、一个金属蛋白酶结构域、一个去整合素结构域富含半胱氨酸结构域、一个表皮生长因子样结构域、一个跨膜结构域和一个胞质结构域。目前报道了ADAM-2的三种亚型，它们在金属蛋白酶结构域和富含半胱氨酸结构域的开始时有所不同。序列由无内含子基因编码。最长的ADAM-2消息编码735个氨基酸的蛋白质，预测质量为82.5 kDa，PI为5.73。ADAM-2的716氨基酸形式在金属蛋白酶结构域开始时相对于较长形式具有缺失，并且预测质量为80.2 kDa和PI为5.75。579个氨基酸的较短形式在MP结构域中共享缺失，并且在富含半胱氨酸的结构域中也有缺失。定位于精子表面上，ADAM-2亚型被认为与ADAM-1（受精素-A）形成异源二聚体，并促进精子卵融合，尽管对于蛋白质的确切作用存在一些争议。整合素A6？卵表面被认为与ADAM-2的整合素结构域中的QDECD基序对接，推测ADAM-1／ADAM-2异源MEDWR在细胞表面产生ADAM-3。