APG4B,自噬相关蛋白4B抗体

产品编号HZ-1384R
英文名称APG4B
中文名称自噬相关蛋白4B抗体
别 名APG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); AUT like 1 cysteine endopeptidase; AUTL1; Autophagin 1; Autophagy related cysteine endopeptidase 1; Autophagy related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; MGC1353br.
规格价格0.1ml 0.2ml
说 明 书0.1ml 0.2ml
研究领域细胞凋亡
抗体来源Rabbit
APG4B,自噬相关蛋白4B抗体克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Cow,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量44kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human APG4B
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
APG4B,自噬相关蛋白4B抗体PubMedPubMed
产品介绍background:
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Function:
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Subcellular Location:
Cytoplasm (Probable).

Tissue Specificity:
Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

Similarity:
Belongs to the peptidase C54 family.

Gene ID:
23192

APG4B,自噬相关蛋白4B抗体Database links:
Entrez Gene: 23192 Human
Entrez Gene: 66615 Mouse
Entrez Gene: 316640 Rat
Omim: 611338 Human
SwissProt: Q9Y4P1 Human
SwissProt: Q8BGE6 Mouse
SwissProt: Q62625 Rat
Unigene: 283610 Human
Unigene: 29087 Mouse
Unigene: 163086 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

细胞自噬（Autophagy）是一种非凋亡性的程序性细胞死亡即自噬性程序性细胞死亡，进化过程中高度保守，清除降解细胞内受损伤的细胞结构、衰老的细胞器、以及不再需要的生物大分子等，在细胞的生长、发育和疾病发生中起着重要的作用。细胞自噬是一个高效调控过程，特异性激酶和鸟嘌呤核苷酸磷酸酶（GTPases）在调节自噬通道活性中起着关键作用.

产品图片

Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-APG4B/AUTL1 Polyclonal Antibody, Unconjugated(bs-1384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining