分子量:65.3 kDa

纯度:>98% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human CD31, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 50000 cells/well are added to Human CD31, His Tag coated plates (3.3 g/mL, 100 l/well) in the presence of 20 ng/mL PMA, approximately 55 - 65% will adhere after 30 minutes at 37°C.

产品特性:Human CD31, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 65.3 kDa. The predicted N-terminus is Gln 28. The reducing (R) protein migrates as 100-110 kDa in SDS-PAGE  due to glycosylation.

产品背景:Cluster of Differentiation 31 (CD31) is also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, T / NK cells, lymphocytes, megakaryocytes, osteoclasts, neutrophils, certain tumors, and is the only known member of the CAM family on platelets. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. CD31 plays a key role in removing aged neutrophils from the body. CD31 mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. In addition, it has been shown that CD31 expression is up-regulated by LPS stimulation, and might function as a feedback negative regulator of LPS inflammatory response in macrophages.