Adeno-associated virus (AAV) is a small virus (20 nm in diameters) replication-defective, nonenveloped virus, which infects humans and some other primate species. AAV is not currently known to cause disease and consequently the virus causes a very mild immune response. AAV can infect both dividing and non-dividing cells and may incorporate its genome into that of the host cell.
With our proprietary genetically engineered AAV·HT™ packaging cell, we are offering shRNA rAAV (conventional single stranded rAAV or double-stranded rAAV also called self-complementary rAAV*) production services for efficient silencing in vitro and in vivo.
If commercial shRNAs or siRNAs of your target gene are not available or non-functional, or your project requires special design for further development, we offer special shRNA design without additional charge!
Advantages:
- One-stop service: from shRNA design to packaging.
- High infection efficiency.
- Quick turnaround time: 2 to 3 weeks.
- Cost-effective.
- Special design request acceptable, FREE!
Wide choice of rAAV cis vectors:
Requirements: GenBank access #, sequence of gene of interest or shRNA is needed.
Deliverables: Total >1.0 ml at >1013 VG/ml** shRNA rAAV for large scale production.
With our proprietary genetically engineered AAV·HT™ packaging cell and a modified rAAV cis plasmid, the shRNA rAAV yield is easy to reach super high level--------total 1E+15 VG.
For super high level rAAV production up to 1E+15 VG , please contact us to request a quote.
* Some terms may apply for custom double-stranded or self-complementary rAAV production service.
** Final viral yield may depend on the transgene in the virus. For rAAV serotype 2, we guarantee final deliverable of >2.0 ml at >1E+12 VG/ml.
shRNA重组AAV病毒定制生产
产品名称： shRNA重组AAV病毒定制生产
英文名称： shRNA AAV Production Service
产品编号：
产品价格： 0
产品产地： null
品牌商标： null
更新时间： null
使用范围： null
济南思科生物科技有限公司
- 联系人 :
- 地址 : 济南市经六路689号绿地·玫瑰坊A座734号
- 邮编 : 250021
- 所在区域 : 山东
- 电话 : 159****9896 点击查看
- 传真 : 点击查看
- 邮箱 : jnqsw@163.com; info@signagen-china.com
shRNA AAV Production Service:
| 型号 | 产品名称- | 价格 |
|---|---|---|
| SL100810 |
shRNA AAV Production, Full Service, Large ScaleFeatures: - Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells. - Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9). - Integration into chromosome 19 of host genome. - Induce efficient gene silencing. Wide Choice of AAV cis Vectors: Service Description: 1. Synthesize and clone shRNA into AAV cis vectors. 2. Large scale transfection of AAV·HT™ 293 cells into 2xcell stack. 3. Harvest rAAV...
|
 ... 更多信息 请询价 |
| SL100871 |
shRNA AAV Production, Full Service, Medium ScaleFeatures: - Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells. - Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9). - Integration into chromosome 19 of host genome. - Induce efficient gene silencing. Wide Choice of AAV cis Vectors: Service Description: 1. Synthesize and clone shRNA into AAV cis vectors. 2. Medium scale transfection of AAV·HT™ 293 cell into 1xcell stack. 3. Harvest rAAV...
|
... 更多信息 请询价 |
| SL100874 |
shRNA AAV Production, Full Service, Pilot ScaleFeatures: - Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells. - Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9). - Integration into chromosome 19 of host genome. - Induce efficient gene silencing. Wide Choice of AAV cis Vectors: Service Description: 1. Synthesize and clone shRNA into AAV cis vectors. 2. Pilot scale transfection of AAV·HT™ 293 cell into 10x150 mm plates. 3. Harvest rAAV...
|
... 更多信息 请询价 |
| SL100872 |
shRNA AAV Packaging, Large ScaleFeatures: - Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells. - Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9). - Integration into chromosome 19 of host genome. - Induce efficient gene silencing. - Wide choice of AAV cis vectors (promoterless, CMV, CAG (also known as CBA), U6, H1, UBC, Synapsin promoters with or without reporter genes like GFP, mRFP, Luc, LacZ, etc). Service Description: -...
|
... 更多信息 请询价 |
| SL100873 |
shRNA AAV Packaging, Medium ScaleFeatures: - Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells. - Multiple serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9). - Integration into chromosome 19 of host genome. - Induce efficient gene silencing. - Wide choice of AAV cis vectors (promoterless, CMV, CAG (also known as CBA), U6, H1, UBC, Synapsin promoters with or without reporter genes like GFP, mRFP, Luc, LacZ, etc). Service Description: -...
|
... 更多信息 请询价 |
| SL100875 |
shRNA Validation ServiceCell-based shRNA Validation Service: - Cloning cDNA of your targeted gene into a validation plasmid - Design and synthesize ~6 shRNA oligoes followed by cloning them to a rAAV cis vector. - Transient co-transfection cDNA/shRNA plasmids. - Quantification of target gene silencing via qRT-PCR Available shRNA Validation Platforms: - Constitutive shRNA knockdown - Inducible shRNA knockdown Guaranteed Knockdown: We guarantee the knockdown of >80% at mRNA level in...
|
... 更多信息 请询价 |